ICSI procedure improve fertilization rate in cases with male factor. But fertilization failure still occurs in 2-3% of ICSI cycles. Literature studies reveal that fertilization failure has been related to sperm, oocyte factor or ICSI technique. But the main cause of failed fertilization is failure to complete oocyte ACTIVATION. Oocyte ACTIVATION includes large number of well defined morphological and biochemical endpoints, some of which occur within seconds or minutes of spermoocyte plasma membrane interaction, and some occur over the course of several hours. Intracellular calcium rise plays an important role in oocyte ACTIVATION. Therefore, researchers have used different methods including mechanical, electrical and chemical to mimic the calcium rise in order to activate the oocytes that will be discussed during the presentation.