Purpose: Chronic periodontitis is the most common form of periodontal disease. The present study assessed the clinical effects of scaling and root planing (SRP) and Er,Cr:YSGG laser for chronic periodontitis treatment.Methods: 16 chronic periodontitis patients were treated with two different methods (SRP and laser). Each method was implemented on one posterior sextant. Clinical parameters including CAL, Bop, PPD, GR, and PI were measured before the intervention.Results: The mean percentage of Bop in SRP and laser groups was 46% and 62%, respectively, one month after the intervention (P<0.05). The laser group's minimum and maximum mean CAL were midlingual and mesiolingual, respectively (P<0.05). The minimum and maximum mean PPD values of the teeth one month after the treatment with the laser were obtained by the midlingual and mesiolingual, respectively. The mean PPD values were significantly different between midlingual and mesiobuccal areas (P<0.05). The mean GR value in SRP and laser group one month after the intervention was 1.63 and 1.19, respectively (P<0.05). The PI in both groups before and after the intervention was similar; however, the differences before and after treatment were significant in both groups.Conclusion: The value of Bop, CAL, PD, and GR decreased in the two groups, and the decrease was more in the laser group. In addition, PI remained unchanged in the two groups before and after the intervention.