Background: Halophilic Archaea based on structural characteristics, physiological, biochemical and genetically distinct from other microorganisms has attracted the attention of scientists. Halobacteriumsalinarum produces a membrane embedded photoprotein with several proposed applications in biotechnology. However, low growth rates of halophilic archaea, often act to hamper all further biotechnological advances. Therefore, to achieve a high rate of growth, culture medium engineering could be affective. One main component of the medium is sodium chloride that its resource and purity may affect the microorganism growth. In this research we compare the effect of two types of the salt on the H. salinarum growth rate.Methods: H. salinarum R1 cultured in a designed reactor with inlet and outlet air tubethat placed in the Shaking incubator at 39oC and 250 rpm. Air provided by a compressor that pumped the filtered stile air through a humidifier into bottom of reactor and exhausted from outlet air tube. Humidifier temperature was equal with growth temperature. Two kind of salts used in this study; first one was purified salt via crystallization procedure. The other one was non purified sea salt from Urmia salt lake. Feeding performed every day and concentration of feeding culture medium components except salt increased depending on the optical density of the microorganism at 600 nm.Results: After 100 hours, OD600 of the microorganism grown in the Medium containing non purified sea salt was as much as 8 whereas OD600 in the case of purified sodium chloride was 4.78 at the same time.Conclusion: It seems that the impurities of the sea salt may have some trace elements that improved aerobic growth of the H. salinarum R1.