IN THE PRESENT WORK, SOME AFFECTED PARAMETERS ON THE LIQUID PHASE MIXING IN THE PROPOSED JET MIXED GAS- LIQUID SYSTEM WERE STUDIED EXPERIMENTALLY. IN THIS TWO-PHASE MIXING SYSTEM, INSTEAD THE IMPELLER IN CONVECTIONAL STIRRED TANKS THE JET FLUID HAS BEEN USED AS A MIXER. IN THE MIXING VESSEL THE JET FLOW AND GAS FLOW ARE COUNTERCURRENT. THE STUDIED PARAMETERS INCLUDED AERATION RATE, JET FLOW RATE AND NOZZLE DIAMETER. THE CREATED FLOW PATTERN EXTRACTED FOR EACH CONDITION AND RESULTS OFTEN ANALYZED ON THE BASIS OF THEM. IT IS OBTAINED, ON THE BASIS OF JET REYNOLDS NUMBER AND THE AMOUNT OF JET TRAVELLING IN THE VESSEL, INCREASING OF AERATION RATE HAVE DIFFERENT EFFECTS ON THE PERFORMANCE OF MIXING. GENERALLY FOUR DIFFERENT TRENDS FOR VARIATION OF MIXING TIME WITH INCREASING OF AERATION RATE ARE OBSERVED. DECREASING OF NOZZLE DIAMETER AT CONSTANT JET FLOW RATE OR INCREASING OF JET FLOW AT CONSTANT NOZZLE DIAMETER CONTINUOUSLY DECREASES THE MIXING TIME. TOO, COMPARISON OF THE JET MIXED SYSTEM RESULTS WITH THE RESULTS OF SIMILAR MECHANICALLY STIRRED SYSTEM SHOWS THE IMPROVING OF LIQUID PHASE MIXING IN THE JET MIXED SYSTEM.

THE DETERMINATION REGION OF SOLUBILITY OF METHANOL WITH GASOLINE OF HIGH AROMATIC CONTENT WAS INVESTIGATED EXPERIMENTALLY AT TEMPERATURE OF 298.2 K. A TYPE 1 LIQUIDLIQUID PHASE DIAGRAM WAS OBTAINED FOR THIS TERNARY SYSTEM. THESE RESULTS WERE CORRELATED SIMULTANEOUSLY BY THE UNIQUAC MODEL. THE VALUES OF THE INTERACTION PARAMETERS BETWEEN EACH PAIR OF COMPONENETS IN THE SYSTEM WERE OBTAINED FOR THE UNIQUAC MODEL USING THE EXPERIMENTAL RESULT. THE ROOT MEAN SQUARE DEVIATION (RMSD) BETWEEN THE OBSERVED AND CALCULATED MOLE PERCENTS WAS 2.86 FOR METHYLCYCLOHEXANE + METHANOL + ETHYLBENZENE. THE MUTUAL SOLUBILITY OF METHYLCYCLOHEXANE AND ETHYLBENZENE WAS ALSO INVESTIGATED BY THE ADDITION OF METHANOL AT 298.2 K.

HYDROCHLOROTHIAZIDE (HCTZ) IS A THIAZIDE DIURETIC AND ANTIHYPERTENSIVE DRUG USED IN THE TREATMENT OF OEDEMA ASSOCIATED WITH CONJESTIVE HEART FAILURE AND RENAL AND HEPATIC DISORDERS [1]. THE AIM OF THE PRESENT INVESTIGATIONS IS TO INTRODUCE LIQUID-LIQUID MICROEXTRACTION WITH HOLLOW FIBERS (HF-LPME) IN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) HAS BEEN APPLIED AS A NEW, FAST, SIMPLE AND EFFICIENT PROCEDURE FOR THE DETERMINATION OF HCTZ IN HUMAN URINE SAMPLE. THE TARGET COMPOUNDS WERE EXTRACTED FROM 24-ML AQUEOUS SAMPLE (DONOR SOLUTION, PH 12.0) THROUGH A MICROFILM OF ORGANIC SOLVENT (1-OCTANOL), IMMOBILIZED IN THE PORES OF A HOLLOW FIBER WITH 8.4 CM LENGTH FIXED ONTO THE TIP OF THE SYRINGE NEEDLE, WHICH WAS DIRECTLY IMMERSED INTO THE SAMPLE [2]. AFTER A PRESCRIBED PERIOD OF TIME, THE ACCEPTOR SOLUTION INSIDE THE FIBER WAS WITHDRAWN INTO THE MICROSYRINGE AND DIRECTLY INJECTED INTO THE HPLC SYSTEM FOR ANALYSIS. FACTORS RELEVANT TO THE EXTRACTION PROCEDURE WERE STUDIED: CHOOSING THE SUITABLE EXTRACTION SOLVENT, PH VALUE OF THE SAMPLE SOLUTION, THE EFFECT OF IONIC STRENGTH (WHICH FOUND THAT THE CONCENTRATION OF NACL SLIGHTLY DECREASES THE EXTRACTION EFFICIENCY), THE STUDY OF ION PAIR EFFECT (ALIQUAT-336 ADDED INTO THE EXTRACTION SOLVENT) [3], IT SHOWED THAT THE SIGNAL OF THE ANALYTE WAS INCREASED BY INCREASING THE CONCENTRATION OF ALIQUAT-336 UP TO 2% (V/V). THE STIRRING OF 800 RPM AND THE EXTRACTION TIME OF 90 MIN WERE CHOSEN AS THE OPTIMUM CONDITIONS.QUANTITATIVE PARAMETERS UNDER THE OPTIMIZED CONDITIONS WERE CALCULATED. LDR OVER THE RANGE OF 1.0-100MG L-1 OF HCTZ, R2 0.9933, LOD 0.5MG L-1, RSD% FOR FOUR REPLICATE MEASUREMENTS OF 25.0MG L-1 WAS 7.4%. UP TO 126-FOLD ENRICHMENT OF ANALYTES WAS DEFINED. IN ORDER TO ASSAY THE APPLICABILITY OF THE NEWLY DEVELOPED EXTRACTION SYSTEM, TAP WATER AND URINE SAMPLES WERE EXTRACTED AND ANALYZED AND MORE THAN 85% RELATIVE RECOVERY OF THE ANALYTE IN URINE SAMPLE WAS ACHIEVED. THE PRESENT STUDY EXHIBITED EXCELLENT PERFORMANCE OF THE CARRIER BASED HF-LPME TECHNIQUE FOR THE ANALYSIS AND EXTRACTION OF TRACE LEVELS OF HCTZ. THE PROPOSED METHOD PROVIDED HIGH PFS FOR THE TARGET ANALYTE, ALONG WITH AN EXCELLENT CLEAN-UP CAPABILITY. THE WHOLE OPERATION IS VERY CONVENIENT TO HANDLE BECAUSE THE RECEIVING PHASE IS PROTECTED BY THE HOLLOW FIBER.