Background: The aim of this study was ethanol production from date palm by yeasts isolated from date palm.Methods: Isolation of yeasts was done according to KIran Sree et al. (2000). For juice preparation from date palm, 100 grams of Zahdi date were mixed with 1 liter distilled water and after mixing them, they were filtered through filter paper. The obtained filtrate was autoclaved in 121oC for 15 min. 1% of 24-hour culture of yeast was used as the inoculum. Determination of ethanol content was done based on dichromate potassium procedure by some modification.Results: In the result 5 isolates including D1, D2, D3, D4 and D5 were isolated from date palm. Among these isolates, D4 was the best isolate and produced 4.9% and 3.2% ethanol in aerobic and anaerobic conditions, respectively. Results of assimilation and fermentation tests of carbohydrates showed that this isolate is Pichia burtonii.Conclusion: In conclusion, this isolate can be used for ethanol production in large scales. Although the ethanol yield is low, maximum being, 4.9% produced by Pichia burtonii D4, the strain can produce more ethanol by optimization of culture conditions.