In a taxonomic study during 2005-2006 on Coccinellid beetles (Col.: Coccinellidae) in Fars province, Iran, a large number of species were collected and identified. Morphological characteristics and male genitalia were used for identification. Professor Fursch from Germany confirmed the identifications.Review of literature indicated that Tytthaspis sedecimpunctata (Linnaeus, 1758) is a new record for Iranian fauna. 6 male and 3 female specimens of this ladybird were collected on wheat plants in 12, May, 2005 from Khafr.This species was recorded from Austria, Belgium, Britain, Bulgaria, Croatia, Czech Republic, Estonia, Finland, Germany, Hungary, Ireland, Italia, Lithuania, Luxembourg, Poland, Romania, Russia, Slovakia, Sweden, Switzerland, Netherlands and North Africa (Fauna Europaea Web Service, 2004; Available online at http://www.faunaeur.org).Tytthaspis Crotch 1874 and Micraspis Dejean, 1836 are synonymies and belong to Coccinellinae Latreille, 1807.T. sedecimpunctata (Linnaeus, 1758) is a small ladybird, 2-3 mm long, yellow in color with 16 black spots on elytra known as Sixteen-spot Ladybird.