Background: Harnessing the potentials of applications (apps) for clinical training and practice has turned into the focal point of innovation. Objectives: The current study was aimed to determine knowledge, perception, and medical apps use among medical students of a Nigerian University. Methods: In this cross-sectional study, 320 medical students (193 males and 127 females) are participated, with a response rate of 90. 9%. The face and content validity was determined by a pilot test on obtained information about knowledge, perception, and use of medical applications, as well as socio-demographic characteristics. Descriptive and inferential statistics were used to analyze the data. Alpha level was set at P < 0. 05. Results: The 12-months and point prevalence of smartphone ownership and using smartphone-based medical apps were 90% and 81. 9%; and 65. 9% and 50. 9%, respectively. There was a high level of knowledge (81. 9%), and positive perception (80%) about medical apps use. Knowledge of medical apps usage was significantly associated with each of age ( 2 = 30. 541, P = 0. 001) and academic levels ( 2 = 19. 270, P = 0. 001). A significant association was found between the perception of medical apps and age ( 2 = 13. 554, P = 0. 004). Also, there was a significant association between current use of medical apps and each of age ( 2 = 12. 018, P = 0. 007) and academic levels ( 2 =11. 297, P = 0. 002). Conclusions: Although smartphone ownership was highamongNigerian medical students, their usage of smartphone-based medical apps was moderate. Being of younger age and higher level of study are major contributors to a high level of knowledge and positive perception about medical apps use.