The purpose of this study was to determine the effectiveness of Brain– based Learning on self-regulation learning among students. This research was carried out in a quasi-experimental and pre-test-post-test design. The statistical population of this research is all female high school students who are in the first year of state high schools in Yazd. The sample consisted of 40 students who were randomly selected from multiple regions and each group (20 subjects) was divided into two experimental and control groups. The learning environment was changed based on the components affecting the brain. During the two 45-minute sessions, students were given the necessary training for brain– based Learning. Courses are also based on the principles of brain– based Learning. Buffer self-regulation learning scale was used to collect data. Data were analyzed using covariance test and paired t-test. The results of variance analysis of covariance showed that brain– based Learning has an effect on self-regulation learning and this effect is stable for 3 months.