Background and Aim: Total antioxidant capacity (TAC) of the tissue is an important factor in determining the physiological or physio-pathological function of oxygen reactive spices. Release of reactive oxygen species (ROS) in dermal wounds, leads to necrosis as well as apoptosis and tissue loss. Management of the risk factors for tissue loss due to flap failure can reduce risk factors with resultant improvement in the survival and efficacy of the flaps. Materials and Methods: 40 Sprauge-Dawkey rats were divided into 4 groups (1) sham group with an 8×3 cm skin cut without skin elevation (2) Flap group with an 8×3 cm skin cut with skin elevation (3) cymophenol group treated with cymophenol (25 mg/kg/day) (4) cymophenol group treated with cymophenol (50 mg/kg/day) after surgery. After one week, percentage of the living tissue, superoxide dismutase (SOD) activity and amount of malondialdehyde (MDA) were measured in the groups and the proximity results were extracted. Using GraphPad Prism 6 software, data were analyzed and compared by ANOVA and Tukey test. Results: Cymophenol application decreased the amount of malondialdehyde (p<0. 05), increased superoxide dismutase activity (p<0. 01), led to reduced oxidative stress in the flap tissues and increased resistance capacity against oxidative stress. Also we found improved survival rate of the flaps (p<0. 05). Conclusion: This study revealed the protective effect of cymophenol against oxidative stress and on survival of the flaps. However, more studies are required to determine its benefits in clinical use.