PILOT PRODUCTION OF TAQ AND PFU DNA POLYMERASES

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MIRZAEI NODUSHAN H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2009 | Volume:17 | Issue:1(33) Page(s): 132-138

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Information Journal Paper

Title

PILOT PRODUCTION OF TAQ AND PFU DNA POLYMERASES

Author(s)

MIRZAEI NODUSHAN H. | Issue Writer Certificate

Keywords

TAQ ENZYMEQ3

PFU ENZYMEQ3

DNA POLYMERASEQ2

GENE TRANSFORMATIONQ3

ENZYME PURIFICATIONQ3

Abstract

Taq and Pfu DNA POLYMERASEs are two important enzymes in molecular biology studies. They are widely used in all molecular biology laboratories. Simple method of production and purification of the two enzymes is reported here to locally supply laboratories with high consumption level. Plasmids containing the Taq and Pfu genes were separately transformed to an E. coli strain. The transformed bacteria were grown on liquid 2x SOB culture medium for 4.5 hours. Bacterial cultures were induced to produce the enzymes. Extraction and purification of the enzymes was conducted as presented in this study. Bradford assay was used to determine the enzyme concentration. The activity and efficiency of Taq and PFU ENZYMEs produced here were compared to a commercial enzyme by amplifying a known genomic fragment of Arabidopsis thaliana ecotype Colombia. Therefore, the enzyme production method adopted in this study was efficient in producing high purity Taq and Pfu.

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APA: Copy

MIRZAEI NODUSHAN, H.. (2009). PILOT PRODUCTION OF TAQ AND PFU DNA POLYMERASES. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 17(1(33)), 132-138. SID. https://sid.ir/paper/104226/en

Vancouver: Copy

MIRZAEI NODUSHAN H.. PILOT PRODUCTION OF TAQ AND PFU DNA POLYMERASES. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2009;17(1(33)):132-138. Available from: https://sid.ir/paper/104226/en

IEEE: Copy

H. MIRZAEI NODUSHAN, “PILOT PRODUCTION OF TAQ AND PFU DNA POLYMERASES,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 17, no. 1(33), pp. 132–138, 2009, [Online]. Available: https://sid.ir/paper/104226/en

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