COMPARISON OF DIFFERENT METHODS FOR ISOLATING OF TOTAL RNA FROM LEAF OF THREE THYME SPECIES RICH IN SECONDARY METABOLITES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BAHADOR S.; RABIEI B.; HASSANI KUMLEH S.H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2014 | Volume:22 | Issue:1 Page(s): 11-24

Download Full-Text

Persian Verion

View:

1,087

Download:

Cites:

Information Journal Paper

Title

COMPARISON OF DIFFERENT METHODS FOR ISOLATING OF TOTAL RNA FROM LEAF OF THREE THYME SPECIES RICH IN SECONDARY METABOLITES

Author(s)

BAHADOR S. | RABIEI B. | HASSANI KUMLEH S.H. | Issue Writer Certificate

Keywords

THYMEQ2

RNA ISOLATIONQ2

METHOD OF SDS-PHENOLQ2

SECONDARY METABOLITESQ2

Abstract

Medicinal plants are rich sources of natural products used for production of pharmaceutical products. Thymus genus belongs to the Lamiaceae family which has more than18 different species distributed in different areas of Iran. Pharmacological effects of THYME are related to phenolic monoterpenes such as carvacrol and thymol. Despite pharmacological effects, the phenolic compounds and other SECONDARY METABOLITES are major problem for isolation of high quality RNA in gene expression analysis and genetic engineering. In the present study, seven different isolation protocols were compared to extract total RNA from three THYME species rich in phenolic SECONDARY METABOLITES in terms of quality and quantity of RNA samples with respect to spectrophotometric reading, denaturing agarose gel electrophoresis, Reverse Transcription Polymerase Chain (RT-PCR) and Real Time-PCR reactions. Three methods of isolation together with two commercial buffers and two kits that were commercially available were used.Results indicated that RNA isolated bymodified SDS- phenol protocol was goodquality and quantity, where standard RNA ISOLATION methods involving GeneJET Plant RNA Purification Mini Kit and TRIZol extraction buffers failed. Finally, we proposed a new suitable protocol for isolating RNA from THYME leaves rich in SECONDARY METABOLITES.

Cites

No record.

References

No record.

Cite

APA: Copy

BAHADOR, S., RABIEI, B., & HASSANI KUMLEH, S.H.. (2014). COMPARISON OF DIFFERENT METHODS FOR ISOLATING OF TOTAL RNA FROM LEAF OF THREE THYME SPECIES RICH IN SECONDARY METABOLITES. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 22(1 ), 11-24. SID. https://sid.ir/paper/104255/en

Vancouver: Copy

BAHADOR S., RABIEI B., HASSANI KUMLEH S.H.. COMPARISON OF DIFFERENT METHODS FOR ISOLATING OF TOTAL RNA FROM LEAF OF THREE THYME SPECIES RICH IN SECONDARY METABOLITES. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2014;22(1 ):11-24. Available from: https://sid.ir/paper/104255/en

IEEE: Copy

S. BAHADOR, B. RABIEI, and S.H. HASSANI KUMLEH, “COMPARISON OF DIFFERENT METHODS FOR ISOLATING OF TOTAL RNA FROM LEAF OF THREE THYME SPECIES RICH IN SECONDARY METABOLITES,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 22, no. 1 , pp. 11–24, 2014, [Online]. Available: https://sid.ir/paper/104255/en

Related Journal Papers