INTERSPECIFIC HYBRIDIZATION BETWEEN POPULUS ALBA L. XP. EUPHRATICA OLIV.USING INVITRO TECHNIQUE

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JAFARI MOFIDABADI A.; MODIR RAHMATI A.R.

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Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2000 | Volume:- | Issue:1 Page(s): 37-52

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Title

INTERSPECIFIC HYBRIDIZATION BETWEEN POPULUS ALBA L. XP. EUPHRATICA OLIV.USING INVITRO TECHNIQUE

Author(s)

JAFARI MOFIDABADI A. | MODIR RAHMATI A.R. | Issue Writer Certificate

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Abstract

An in vitro method was used to produce hybrid poplar plants between Populus alba L. and P. euphratica OLIV in two directions. Developed ovaries and ovules were isolated from P. alba L. female branches, Which had been, pollinated with P. euphratica pollen grains by using twig and pot breeding technique. The same procedure was carried out for isolation of developed P. euphratica OLIV ovaries and ovules which had been pollinated with Populus alba L. pollen grain on mature trees. Isolated ovaries and ovules of both crosses were then transferred to growth regulator free half concentrated MS agar medium for embryo germination. Fourteen days old Populus alba L. ovary and ovule embryos produced the highest number of plantlets, While 45 days old ovary and ovule embryos of P. euphratica OLIV were necessary to obtain hybrid plants. Maximum two to three plantlets were observed per both Populusalba L. and P. euphratica OLIV ovary culture. Ninety percent of pollinated P. euphratica OLIV and Populus alba L. ovules successfully produced plantlets, whereas the efficiency of pollinated ovaries to produce plantlets was 70% for Populus alba L. and 67% for P. euphratica OLIV.Plantlets were cultured in the same medium in jars, before being transferred to potting soil. Seventy-five interspecific P. alba L. x P. euphratica OLIV. and twenty-six of P. euphratica OLIV. x Populus alba L. hybrid plants were successfully acclimatized in greenhouse.

Cites

HYBRIDIZATION BETWEEN INDIGENOUS AND EXOTIC POPLAR CLONES

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APA: Copy

JAFARI MOFIDABADI, A., & MODIR RAHMATI, A.R.. (2000). INTERSPECIFIC HYBRIDIZATION BETWEEN POPULUS ALBA L. XP. EUPHRATICA OLIV.USING INVITRO TECHNIQUE. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, -(1), 37-52. SID. https://sid.ir/paper/104296/en

Vancouver: Copy

JAFARI MOFIDABADI A., MODIR RAHMATI A.R.. INTERSPECIFIC HYBRIDIZATION BETWEEN POPULUS ALBA L. XP. EUPHRATICA OLIV.USING INVITRO TECHNIQUE. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2000;-(1):37-52. Available from: https://sid.ir/paper/104296/en

IEEE: Copy

A. JAFARI MOFIDABADI, and A.R. MODIR RAHMATI, “INTERSPECIFIC HYBRIDIZATION BETWEEN POPULUS ALBA L. XP. EUPHRATICA OLIV.USING INVITRO TECHNIQUE,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. -, no. 1, pp. 37–52, 2000, [Online]. Available: https://sid.ir/paper/104296/en

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