Isolation, characterization and expression analysis of 3-hydroxy-N-methylcoclaurine 4′ – methyltransferase (4'OMT2) gene in Papaver somniferum L.

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SOHRABI S.M.; ISMAILI A.; NAZARIAN FIROUZ ABADI F.; SAMIEI K.

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2019 | Volume:26 | Issue:2 (52) Page(s): 165-176

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

524

Download:

Cites:

Information Journal Paper

Title

Isolation, characterization and expression analysis of 3-hydroxy-N-methylcoclaurine 4′ – methyltransferase (4'OMT2) gene in Papaver somniferum L.

Author(s)

SOHRABI S.M. | ISMAILI A. | NAZARIAN FIROUZ ABADI F. | SAMIEI K. | Issue Writer Certificate

Keywords

Gene expressionQ2

Genomic sequenceQ1

PoppyQ1

SRAQ1

3′-hydroxy-Nmethylcoclaurine 4′-O-methyltransferaseQ1

Abstract

Poppy (Papaver somniferum L. ) is one of the oldest medicinal plant in the world. It produces several important narcotic benzylisoquinoline alkaloids. In this study, 4’ OMT2 (3′-hydroxy-Nmethylcoclaurine 4′-O-methyltransferase) gene was isolated, sequenced and characterized. After amplification of genomic and coding sequence of 4’ OMT2 gene using PCR, the fragments were cloned into pTZ57R/T plasmid and sequenced. Sequencing results showed two fragments of 1074 and 1189bp for coding and Genomic sequences, respectively. For the first time, results showed one intron of 115bp in the Genomic sequence of 4’ OMT2 in the species. Isolated gene from Iranian genotypes was similar to Papaver genus with high identity, and had 67% identity to Ranunculales plants. Further bioinformatics analyses revealed, 4’ OMT2 gene produces a stable enzyme without signal peptide that localize in the cytoplasm. For Gene expression analysis, five Poppy RNA-seq (mRNA type) libraries (belonging to flower bud, leaf, developing fruit, stem and root tissues) were retrieved from SRA database and analysis of 4’ OMT2 Gene expression were done using IDEG6 software and statistical tests. Results showed that 4’ OMT2 has differential expression among different tissues and has maximum expression in stem tissues. Identification and characterization of biosynthetic genes is the first step in genetic manipulation and metabolic engineering. In present study, for the first time, full length coding sequence of 4’ OMT2 gene was isolated from Poppy plant and presence of one intron in its sequence was determined.

Cites

No record.

References

No record.

Cite

APA: Copy

SOHRABI, S.M., ISMAILI, A., NAZARIAN FIROUZ ABADI, F., & SAMIEI, K.. (2019). Isolation, characterization and expression analysis of 3-hydroxy-N-methylcoclaurine 4′ – methyltransferase (4'OMT2) gene in Papaver somniferum L.. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 26(2 (52) ), 165-176. SID. https://sid.ir/paper/104492/en

Vancouver: Copy

SOHRABI S.M., ISMAILI A., NAZARIAN FIROUZ ABADI F., SAMIEI K.. Isolation, characterization and expression analysis of 3-hydroxy-N-methylcoclaurine 4′ – methyltransferase (4'OMT2) gene in Papaver somniferum L.. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2019;26(2 (52) ):165-176. Available from: https://sid.ir/paper/104492/en

IEEE: Copy

S.M. SOHRABI, A. ISMAILI, F. NAZARIAN FIROUZ ABADI, and K. SAMIEI, “Isolation, characterization and expression analysis of 3-hydroxy-N-methylcoclaurine 4′ – methyltransferase (4'OMT2) gene in Papaver somniferum L.,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 26, no. 2 (52) , pp. 165–176, 2019, [Online]. Available: https://sid.ir/paper/104492/en

Related Journal Papers