STUDY OF TISSUE CULTURE IN DIOECIOUS SORREL

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SHAKIB A.M.; AHMAD RAJI A.; OROUJLOU M.; IZADI DARBANDI A.

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Journal Paper

Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2008 | Volume:15 | Issue:4 (30) Page(s): 273-283

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Information Journal Paper

Title

STUDY OF TISSUE CULTURE IN DIOECIOUS SORREL

Author(s)

SHAKIB A.M. | AHMAD RAJI A. | OROUJLOU M. | IZADI DARBANDI A. | Issue Writer Certificate

Keywords

SORREL (RUMEX ACETOSA L)Q3

EXPLANTQ2

CALLUS INDUCTION AND REGENERATION TISSUE CULTUREQ3

Abstract

Sorrel (Rumex acetosa L.) is a perennial species, being used as a model plant in molecular genetic studies of sex determination, because of several characteristics such as dioecy, sex chromosomes and short life cycle it is In this study regeneration potentials of different tissues such as cotyledon, hypocotyl, leaf, petiole and flowering stem cultured on media containing different cytokinin and auxins combinations are presented. The results showed callus induction in cultured tissues on media containing auxins. Tissues cultured on media containing cytokinins alone or in combinations with auxins showed regeneration response. The highest regeneration rate 100% was obtained from stem segments cultured on medium containing 1 mg/l TDZ. Leaf and petiole after stem had regeneration rate of 35% and 40 % on medium containing 1 mg/l TDZ and 0.75 mg/l IAA and NAA. The highest number of regenerates per EXPLANTs was 8.8 using stem segments cultured on medium containing TDZ. Histological observations showed that regenerated shoots originated from the cells in the area of the vascular tissues. Regenerated shoots rooted on medium without hormone and plants produced seeds after growth in glasshouse conditions.

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References

HIGH FREQUENCY PLANTLET REGENERATION IN A DIOECIOUS PLANT AS SORREL

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APA: Copy

SHAKIB, A.M., AHMAD RAJI, A., OROUJLOU, M., & IZADI DARBANDI, A.. (2008). STUDY OF TISSUE CULTURE IN DIOECIOUS SORREL. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 15(4 (30)), 273-283. SID. https://sid.ir/paper/104639/en

Vancouver: Copy

SHAKIB A.M., AHMAD RAJI A., OROUJLOU M., IZADI DARBANDI A.. STUDY OF TISSUE CULTURE IN DIOECIOUS SORREL. IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2008;15(4 (30)):273-283. Available from: https://sid.ir/paper/104639/en

IEEE: Copy

A.M. SHAKIB, A. AHMAD RAJI, M. OROUJLOU, and A. IZADI DARBANDI, “STUDY OF TISSUE CULTURE IN DIOECIOUS SORREL,” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 15, no. 4 (30), pp. 273–283, 2008, [Online]. Available: https://sid.ir/paper/104639/en

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