EFFECTS OF SEVERAL PLANT GROWTH REGULATORS, CARBON SOURCE AND AMINO ACIDS ON SOMATIC EMBRYOS OBTAINED FROM EMBRYOGENIC CELL SUSPENSION CULTURE OF ALFALFA (MEDICAGO SATIVA L.)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

PEZESHKI ATEFEH; EBRAHIMI MORTEZA; KHAYAM NEKOUEI M.; KADKHODAEI SAEID

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research Year:2010 | Volume:18 | Issue:1 (35) Page(s): 63-73

Download Full-Text

Persian Verion

View:

1,292

Download:

Cites:

Information Journal Paper

Title

EFFECTS OF SEVERAL PLANT GROWTH REGULATORS, CARBON SOURCE AND AMINO ACIDS ON SOMATIC EMBRYOS OBTAINED FROM EMBRYOGENIC CELL SUSPENSION CULTURE OF ALFALFA (MEDICAGO SATIVA L.)

Author(s)

PEZESHKI ATEFEH | EBRAHIMI MORTEZA | KHAYAM NEKOUEI M. | KADKHODAEI SAEID | Issue Writer Certificate

Keywords

ALFALFAQ3

B5 CULTURE MEDIUMQ2

SOMATIC EMBRYOGENESISQ3

CELL SUSPENSION CULTUREQ3

GROWTH REGULATORQ3

Abstract

ALFALFA (Medicago sativa L.) is one of the most important forage crops in the world that have a significant role in forage supplying, nitrogen fixation and reduction of soil erosion. The species is self-incompatible and cross pollinate, so maintenance and propagation of desired genotypes are difficult and production of pure and hemogeneous seeds is faced with difficulties. SOMATIC EMBRYOGENESIS is one of the most useful techniques that can help us to solve the problems. Therefore, recognition of the affecting factors on SOMATIC EMBRYOGENESIS is important. This study was aimed to evaluate the effects of two GROWTH REGULATORs, 2,4-D (0.5 and 1 mgL-1) and Kinetin (0 and 1 mgL-1), carbon source (sucrose and maltose) and Glutamine and Serine as amino acids, on ALFALFA somatic embryogensis in an embryogenic CELL SUSPENSION CULTURE using 4 explants (hypocotyl, cotyledon, petiole and leaf). The treatments showed significant differences. There was a correlation between 2,4-D concentration and the number of embryos in maturity stage, so that increasing the concentration of 2,4-D resulted in lower number of embryos. Presence of Kinetin in the culture media increased number of somatic embryos, significantly. Despite significant effects of Glutamine and Serine on embryo development, application of these amino acids in suspension culture media decreased the regeneration of somatic embryos in the liquid cultures. Maltose in liquid culture was the most effective agent on the maturity of somatic embryos and increased the number of embryos in embryogenic CELL SUSPENSION CULTURE. Four types of the examined explants, showed significant differences of embryogenic ability; hypocotyl and petiole explants produced the most and the least number of embryos, respectively.

Cites

No record.

References

No record.

Cite

APA: Copy

PEZESHKI, ATEFEH, EBRAHIMI, MORTEZA, KHAYAM NEKOUEI, M., & KADKHODAEI, SAEID. (2010). EFFECTS OF SEVERAL PLANT GROWTH REGULATORS, CARBON SOURCE AND AMINO ACIDS ON SOMATIC EMBRYOS OBTAINED FROM EMBRYOGENIC CELL SUSPENSION CULTURE OF ALFALFA (MEDICAGO SATIVA L.). IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, 18(1 (35)), 63-73. SID. https://sid.ir/paper/104643/en

Vancouver: Copy

PEZESHKI ATEFEH, EBRAHIMI MORTEZA, KHAYAM NEKOUEI M., KADKHODAEI SAEID. EFFECTS OF SEVERAL PLANT GROWTH REGULATORS, CARBON SOURCE AND AMINO ACIDS ON SOMATIC EMBRYOS OBTAINED FROM EMBRYOGENIC CELL SUSPENSION CULTURE OF ALFALFA (MEDICAGO SATIVA L.). IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH[Internet]. 2010;18(1 (35)):63-73. Available from: https://sid.ir/paper/104643/en

IEEE: Copy

ATEFEH PEZESHKI, MORTEZA EBRAHIMI, M. KHAYAM NEKOUEI, and SAEID KADKHODAEI, “EFFECTS OF SEVERAL PLANT GROWTH REGULATORS, CARBON SOURCE AND AMINO ACIDS ON SOMATIC EMBRYOS OBTAINED FROM EMBRYOGENIC CELL SUSPENSION CULTURE OF ALFALFA (MEDICAGO SATIVA L.),” IRANIAN JOURNAL OF RANGELANDS AND FORESTS PLANT BREEDING AND GENETIC RESEARCH, vol. 18, no. 1 (35), pp. 63–73, 2010, [Online]. Available: https://sid.ir/paper/104643/en

Related Journal Papers