Phytochemical investigation of chloroform extract of Eryngium noeanum Boiss.

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GHASEMI S.; HABIBI Z.; Rezaalizadeh Rooshan f.

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ISSN: 2588-4824

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Journal: IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS Year:2019 | Volume:34 | Issue:6 (92) Page(s): 1035-1047

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Title

Phytochemical investigation of chloroform extract of Eryngium noeanum Boiss.

Author(s)

GHASEMI S. | HABIBI Z. | Rezaalizadeh Rooshan f. | Issue Writer Certificate

Keywords

UmbelliferaeQ2

Eryngium noeanum Boiss.Q1

β-stigmasterolQ1

xanthotoxinQ1

Abstract

In this study, the chloroform extract of the aerial parts of Eryngium noeanum Boiss. from Umbelliferae family was investigated for the first time. The aerial parts of this plant were collected at the flowering stage from Estahban, Fars Province, Iran. The extract was fractionated by the gravity column chromatography on silica gel with a gradient n-hexane, n-hexane-ethyl acetate, ethyl acetate, ethyl acetate– methanol, and methanol as eluent. The similar fractions were combined according to TLC analysis and eight fractions were collected. Two known compounds namely β-stigmasterol (1) and xanthotoxin (2) were isolated. β-stigmasterol was extracted from fraction 3 by thin layer chromatography in n-hexane: ethyl acetate (6: 1, 30 mg). The sixth fraction was purified using repeated column chromatography and thin layer chromatography in n-hexane: ethyl acetate as the eluent (4: 1) to obtain the known coumarin 2 (8 mg). The structure of compounds was determined by their comprehensive spectroscopic analysis including EI-MS, 1H NMR, 13C NMR, H-H COSY, DEPT, HMQC, and comparison with the literature data.

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APA: Copy

GHASEMI, S., HABIBI, Z., & Rezaalizadeh Rooshan, f.. (2019). Phytochemical investigation of chloroform extract of Eryngium noeanum Boiss.. IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS, 34(6 (92) ), 1035-1047. SID. https://sid.ir/paper/105111/en

Vancouver: Copy

GHASEMI S., HABIBI Z., Rezaalizadeh Rooshan f.. Phytochemical investigation of chloroform extract of Eryngium noeanum Boiss.. IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS[Internet]. 2019;34(6 (92) ):1035-1047. Available from: https://sid.ir/paper/105111/en

IEEE: Copy

S. GHASEMI, Z. HABIBI, and f. Rezaalizadeh Rooshan, “Phytochemical investigation of chloroform extract of Eryngium noeanum Boiss.,” IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS, vol. 34, no. 6 (92) , pp. 1035–1047, 2019, [Online]. Available: https://sid.ir/paper/105111/en

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