Effects of auxins on induction and establishment of adventitious and hairy roots culture of the medicinal plant chicory (Cichorium intybus L. )

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HADIZADEH H.; MOHEBODINI M.; Esmaeilpoor B.

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Paper Information

Journal: IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS Year:2016 | Volume:32 | Issue:3 (77) Page(s): 389-397

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Title

Effects of auxins on induction and establishment of adventitious and hairy roots culture of the medicinal plant chicory (Cichorium intybus L. )

Author(s)

HADIZADEH H. | MOHEBODINI M. | Esmaeilpoor B. | Issue Writer Certificate

Keywords

MediumQ2

fresh weightQ1

dry weightQ1

Indole-3-acetic acidQ1

Indole-3-butyric acidQ2

Naphthalene acetic acidQ2

Abstract

Roots are the site for biosynthesis or accumulation of major plant secondary metabolites. In recent decades, many researchers have focused on the biosynthesis of valuable secondary metabolites in hairy roots. This study was conducted to optimize the induction and the culture conditions of adventitious and hairy roots of chicory (Cichorium intybus L. ) using auxins IAA, IBA and NAA. This study was performed in two steps. In the first step, the leaf explant was cultured on MS solid Medium for root induction with different concentrations of IAA and NAA (0, 0. 3, 0. 6 and 1 mg 1-1). The highest root induction (100 %) and the highest mean number of roots were obtained at concentration of 1 mg 1-1 NAA. In the second step, the adventitious and hairy roots obtained from leaf explants were cultured in MS liquid Medium with different concentrations of IAA, IBA and NAA (0, 0. 5, 1 and 1. 5 mg 1-1). According to the results, the highest fresh and dry weight was obtained in MS liquid Medium containing 0. 3 mg 1-1 NAA and 1. 5 mg 1-1 IBA and hairy roots were only established in treatments containing NAA hormone.

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APA: Copy

HADIZADEH, H., MOHEBODINI, M., & Esmaeilpoor, B.. (2016). Effects of auxins on induction and establishment of adventitious and hairy roots culture of the medicinal plant chicory (Cichorium intybus L. ). IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS, 32(3 (77) ), 389-397. SID. https://sid.ir/paper/105214/en

Vancouver: Copy

HADIZADEH H., MOHEBODINI M., Esmaeilpoor B.. Effects of auxins on induction and establishment of adventitious and hairy roots culture of the medicinal plant chicory (Cichorium intybus L. ). IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS[Internet]. 2016;32(3 (77) ):389-397. Available from: https://sid.ir/paper/105214/en

IEEE: Copy

H. HADIZADEH, M. MOHEBODINI, and B. Esmaeilpoor, “Effects of auxins on induction and establishment of adventitious and hairy roots culture of the medicinal plant chicory (Cichorium intybus L. ),” IRANIAN JOURNAL OF MEDICINAL AND AROMATIC PLANTS, vol. 32, no. 3 (77) , pp. 389–397, 2016, [Online]. Available: https://sid.ir/paper/105214/en

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