MAPPING OF THE GENES CONTROLING COCOON SHELL PERCENTAGE TRAIT IN SILKWORM (BOMBYX MORI L.) USING AFLP MARKERS

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MIRHOSSEINI S.Z.; BIZHANNIA A.R.; RABIEI B.; TAEB M.; SEIDAVI A.R.; POTKI P.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2012 | Volume:7 | Issue:1 (28) Page(s): 25-35

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Title

MAPPING OF THE GENES CONTROLING COCOON SHELL PERCENTAGE TRAIT IN SILKWORM (BOMBYX MORI L.) USING AFLP MARKERS

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Keywords

AFLPQ2

COMPOSITE INTERVAL MAPPINGQ2

LINKAGE MAPQ2

MARKERQ2

SILKWORMQ2

Abstract

selection method using DNA MARKERs is an assured method in breeding process via preparation of LINKAGE MAP and QTLs determination of effective genes on quantitative traits control. This method can be used in the SILKWORM, since it has economical-productive and polygenic traits. It was used in this research for determination of QTLs of shell cocoon percentage by AFLP MARKERs, 20 compounds of two primers (TaqI & PstI) that selected from 81 compounds in 33, 36 and 34 of F2 individuals of three mulberry SILKWORM populations which these progency was produced by mating of two Lemon Khorasan (as female) and 107 (as male) lines as pure parents. The extraction of DNA was done using phenolchloroform and extracted DNA was digested by TaqI and PstI restriction enzymes, so they linked to suitable adaptors and selected propagation of all samples of DNA was done by means of all of the primer compounds. The propagated samples were transferred to the acrylamid gel 6% and the LINKAGE MAPs of studied populations were drawn based on matrix formation (number of progency of F2 population× number of appeared poly-morphic bands) by Map Manager and Cartographer ver.2.5 Softwares after genotyping of individuals and ultimately is identified number of possible locations of studied trait controller based on QTL analysis through COMPOSITE INTERVAL MAPPING in Likelihood Ratio Test (LRT) >13 and detected 4, 2 and 5 QTLs respectively within recessive-overdominance limitation.

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APA: Copy

MIRHOSSEINI, S.Z., BIZHANNIA, A.R., RABIEI, B., TAEB, M., SEIDAVI, A.R., & POTKI, P.. (2012). MAPPING OF THE GENES CONTROLING COCOON SHELL PERCENTAGE TRAIT IN SILKWORM (BOMBYX MORI L.) USING AFLP MARKERS. MODERN GENETICS JOURNAL (MGJ), 7(1 (28)), 25-35. SID. https://sid.ir/paper/116473/en

Vancouver: Copy

MIRHOSSEINI S.Z., BIZHANNIA A.R., RABIEI B., TAEB M., SEIDAVI A.R., POTKI P.. MAPPING OF THE GENES CONTROLING COCOON SHELL PERCENTAGE TRAIT IN SILKWORM (BOMBYX MORI L.) USING AFLP MARKERS. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2012;7(1 (28)):25-35. Available from: https://sid.ir/paper/116473/en

IEEE: Copy

S.Z. MIRHOSSEINI, A.R. BIZHANNIA, B. RABIEI, M. TAEB, A.R. SEIDAVI, and P. POTKI, “MAPPING OF THE GENES CONTROLING COCOON SHELL PERCENTAGE TRAIT IN SILKWORM (BOMBYX MORI L.) USING AFLP MARKERS,” MODERN GENETICS JOURNAL (MGJ), vol. 7, no. 1 (28), pp. 25–35, 2012, [Online]. Available: https://sid.ir/paper/116473/en

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