STUDY OF GENETIC DIVERSITY IN AEGHILOPS TAUSCHII ACCESSIONS USING SSR MARKER

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ESMAILI K.; MEHRABI A.A.; ETMINAN A.R.; AZIZIAN E.; MANSOURY S.; HOSSEIN ABADI M.; HAIDARNEZHADIAN M.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2013 | Volume:7 | Issue:4 (31) Page(s): 333-342

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Title

STUDY OF GENETIC DIVERSITY IN AEGHILOPS TAUSCHII ACCESSIONS USING SSR MARKER

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Keywords

AEGILOPS TAUSCHIIQ2

GENETIC VARIATIONQ2

POLYMORPHISMQ2

SSR MARKERQ2

WHEATQ2

Abstract

Wheat has known as the most important cereal in world’s agriculture which has provided over to 30% of cereals of the world. GENETIC VARIATION is too important as the leaven of plant breeding. Like many other crops, the GENETIC VARIATION is diminishing also in the WHEAT. Therefor, plant breeders are looking for the new gene sources they proposed the WHEAT native varieties its wild relatives as a potential useful valuable source of GENETIC VARIATION which may applied on WHEAT breeding successfully. In this study, GENETIC VARIATION of 30 genotypes of Agilops tauschii sp. Were investigated using SSR (Microsatellite) marker. Here, after the DNA extraction based on modified CTAB method quantitative qualitative evaluation of extracted DNA, DNA amplification (PCR) was carried out. Next, electrophoresis of amplified DNA on 3% agarose gelling agent was performed after the band appearing data analysis of GENETIC VARIATION, number of 71 allels were recognized that 69 allels of them found to be polymorphic. Total mean of allel per locus was 4.6 mean value of 0.654 was calculated for POLYMORPHISM contents. The highest degree of dissimilarity was observed from 3 12 the lowest from 7 10 genotypes. The highest the lowest genetic gap of 0.85 0.265 respectively was obtained from dice of 0.92 0.42 from jacard method. Evaluation of dissimilarity between genotypes showed that northern regions of iran specially mazandaran province is the variety center the main origin of Agilops tauschii sp. this species has transferred from these regions to other places.

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APA: Copy

ESMAILI, K., MEHRABI, A.A., ETMINAN, A.R., AZIZIAN, E., MANSOURY, S., HOSSEIN ABADI, M., & HAIDARNEZHADIAN, M.. (2013). STUDY OF GENETIC DIVERSITY IN AEGHILOPS TAUSCHII ACCESSIONS USING SSR MARKER. MODERN GENETICS JOURNAL (MGJ), 7(4 (31)), 333-342. SID. https://sid.ir/paper/116583/en

Vancouver: Copy

ESMAILI K., MEHRABI A.A., ETMINAN A.R., AZIZIAN E., MANSOURY S., HOSSEIN ABADI M., HAIDARNEZHADIAN M.. STUDY OF GENETIC DIVERSITY IN AEGHILOPS TAUSCHII ACCESSIONS USING SSR MARKER. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2013;7(4 (31)):333-342. Available from: https://sid.ir/paper/116583/en

IEEE: Copy

K. ESMAILI, A.A. MEHRABI, A.R. ETMINAN, E. AZIZIAN, S. MANSOURY, M. HOSSEIN ABADI, and M. HAIDARNEZHADIAN, “STUDY OF GENETIC DIVERSITY IN AEGHILOPS TAUSCHII ACCESSIONS USING SSR MARKER,” MODERN GENETICS JOURNAL (MGJ), vol. 7, no. 4 (31), pp. 333–342, 2013, [Online]. Available: https://sid.ir/paper/116583/en

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