OVEREXPRESSION OF TYDC2 IN MEDICINAL PLANT OPIUM POPPY TO INCREASE ITS MEDICINAL ALKALOIDS CONTENTS

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KOOHZADI F.; OMIDI M.; SOLOUKI K.; MAHDINEJAD N.; ALIZADEH H.

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Journal: MODERN GENETICS JOURNAL (MGJ) Year:2013 | Volume:7 | Issue:4 (31) Page(s): 343-352

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Title

OVEREXPRESSION OF TYDC2 IN MEDICINAL PLANT OPIUM POPPY TO INCREASE ITS MEDICINAL ALKALOIDS CONTENTS

Author(s)

Keywords

KOZAK SEQUENCEQ2

MORPHINEQ2

OVEREXPRESSIONQ2

PAPAVER SOMNIFERUMQ2

TYROSINE DECARBOXYLASE (TYDC2)Q2

Abstract

Morphinan group alkaloids in poppy are synthesized from two L-Tyrosine amino acids by 17 anzymatic stages through several continuous oxidative reactions. The first key enzyme on this path is L-Tyrosine decarboxylase (TYDC). Tyrosine decarboxylase is a common cyclic amino acid in plants which in different plant species, biosynthesis of a large number of primary and secondary metabolites depends on it. For over expression of this gene in opium poppy, a suitable gene construct named pBI121-TK was designed, so that Kozak Enhancer as a gene expression enhancer was added at upstream of TYDC2 gene’s start codon and was coloned in pTZ57R/T plasmid. Then, in GUS location under control of CaMV35S promoter and Nos terminator, it entered T-DNA area of pBI121 binary plasmid. Gene transfer optimization by GUS histochemical assay in agroinfiltrated plants was done by pBI121 plasmid containing GUS gene and eventually the agrobacterium containing pBI121-TK recombinant construct was transferred to six-week long plants according to the optimized condition. Amount of morphinan alkaloids was measured by HPLC device. The obtained results indicate 75.2% increase of MORPHINE, codein and thebaine in recombinant sample relative to the control sample.

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APA: Copy

KOOHZADI, F., OMIDI, M., SOLOUKI, K., MAHDINEJAD, N., & ALIZADEH, H.. (2013). OVEREXPRESSION OF TYDC₂ IN MEDICINAL PLANT OPIUM POPPY TO INCREASE ITS MEDICINAL ALKALOIDS CONTENTS. MODERN GENETICS JOURNAL (MGJ), 7(4 (31)), 343-352. SID. https://sid.ir/paper/116585/en

Vancouver: Copy

KOOHZADI F., OMIDI M., SOLOUKI K., MAHDINEJAD N., ALIZADEH H.. OVEREXPRESSION OF TYDC₂ IN MEDICINAL PLANT OPIUM POPPY TO INCREASE ITS MEDICINAL ALKALOIDS CONTENTS. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2013;7(4 (31)):343-352. Available from: https://sid.ir/paper/116585/en

IEEE: Copy

F. KOOHZADI, M. OMIDI, K. SOLOUKI, N. MAHDINEJAD, and H. ALIZADEH, “OVEREXPRESSION OF TYDC₂ IN MEDICINAL PLANT OPIUM POPPY TO INCREASE ITS MEDICINAL ALKALOIDS CONTENTS,” MODERN GENETICS JOURNAL (MGJ), vol. 7, no. 4 (31), pp. 343–352, 2013, [Online]. Available: https://sid.ir/paper/116585/en

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