MOLECULAR IDENTIFICATION OF ACTINOMYCETES IN THE BUTTON MUSHROOM COMPOST USING ANALYSIS OF 16S RRNA GENE RESTRICTION FRAGMENT PATTERNS

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FARSI M.; PAKDIN PARIZI A.; MALEKZADEH KH.

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Paper Information

Journal: MODERN GENETICS JOURNAL (MGJ) Year:2013 | Volume:8 | Issue:2 (33) Page(s): 139-148

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Title

MOLECULAR IDENTIFICATION OF ACTINOMYCETES IN THE BUTTON MUSHROOM COMPOST USING ANALYSIS OF 16S RRNA GENE RESTRICTION FRAGMENT PATTERNS

Author(s)

FARSI M. | PAKDIN PARIZI A. | MALEKZADEH KH. | Issue Writer Certificate

Keywords

ACTINOMYCETESQ2

AGARICUS BISPORUSQ2

ARDRAQ2

COMPOSTQ2

16S RRNA GENEQ2

Abstract

Thermophilic ACTINOMYCETES due to their ability to decompose the cellulolytic materials are of the main components of COMPOST micro-flora of the button mushroom. Different methods including morphological, biochemical and molecular techniques have been used to separate the ACTINOMYCETES into different groups. PCR-based methods such as the pattern of amplified rDNA restriction analysis (ARDRA) have been shown to be useful in differentiating between bacterial species within a genus. Comparison of restricted fragments patterns from isolated bacteria with a database containing 16S RRNA GENE sequences of all validly published filamentous from GenBank using In silico digestions is a rapid method for identification of ACTINOMYCETES. Thermophilic ACTINOMYCETES of COMPOST were isolated on culture medium using dilution methods and incubation at 46oC. The 16S RRNA GENE of each isolates were amplified by PCR and digested by restriction endonucleases. The restricted pattern for each enzyme was determined by electrophoresis. Unknown isolates were identified using amplified rDNA restriction analysis. Most isolated ACTINOMYCETES from the white button mushroom COMPOST belonged to Streptomyces genus which has a significant role in degradation of plant residuals and COMPOST production.

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APA: Copy

FARSI, M., PAKDIN PARIZI, A., & MALEKZADEH, KH.. (2013). MOLECULAR IDENTIFICATION OF ACTINOMYCETES IN THE BUTTON MUSHROOM COMPOST USING ANALYSIS OF 16S RRNA GENE RESTRICTION FRAGMENT PATTERNS. MODERN GENETICS JOURNAL (MGJ), 8(2 (33)), 139-148. SID. https://sid.ir/paper/116605/en

Vancouver: Copy

FARSI M., PAKDIN PARIZI A., MALEKZADEH KH.. MOLECULAR IDENTIFICATION OF ACTINOMYCETES IN THE BUTTON MUSHROOM COMPOST USING ANALYSIS OF 16S RRNA GENE RESTRICTION FRAGMENT PATTERNS. MODERN GENETICS JOURNAL (MGJ)[Internet]. 2013;8(2 (33)):139-148. Available from: https://sid.ir/paper/116605/en

IEEE: Copy

M. FARSI, A. PAKDIN PARIZI, and KH. MALEKZADEH, “MOLECULAR IDENTIFICATION OF ACTINOMYCETES IN THE BUTTON MUSHROOM COMPOST USING ANALYSIS OF 16S RRNA GENE RESTRICTION FRAGMENT PATTERNS,” MODERN GENETICS JOURNAL (MGJ), vol. 8, no. 2 (33), pp. 139–148, 2013, [Online]. Available: https://sid.ir/paper/116605/en

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