DETECTION OF AVIAN INFLUENZA VIRUS OF H9 SUBTYPE IN THE FECES OF EXPERIMENTALLY INFECTED CHICKENS BY RT-PCR

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NOUROUZIAN H.; VASFI MARANDI M.

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Journal: ARCHIVES OF RAZI INSTITUTE Year:2007 | Volume:62 | Issue:4 Page(s): 181-189

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Title

DETECTION OF AVIAN INFLUENZA VIRUS OF H9 SUBTYPE IN THE FECES OF EXPERIMENTALLY INFECTED CHICKENS BY RT-PCR

Author(s)

NOUROUZIAN H. | VASFI MARANDI M. | Issue Writer Certificate

Keywords

AVIAN INFLUENZAQ4

RT-PCRQ4

BROILER CHIBKENSQ3

Abstract

AVIAN INFLUENZA (AI) is a viral respiratory disease of domestic and wild birds. In the diagnostic laboratory, it is essential to have methods for rapid detection of avian respiratory viruses. Cloacal swabs collected from chickens experimentally infected with H9 subtype AI virus, used in a reverse transcription-polymerase chain reaction (RT-PCR) assay for detection of AI. In infected animals, AI viruses are detected most trequently between days 3 and 7 post infection (p.i.). The RT-PCR assay was able to detect, at least, 103.5 EID50 of AI viruses in the allantoic fluid. The RT-PCR assay did not show any cross-reactivity with some other avian respiratory viruses. In comparison with virus isolation (VI) assay, the relative sensitivity, specificity, correlation rate and positive predictive value of the RT-PCR were 80%, 84%, 82% and 83%, respectively. The Kindex of agreement between the two tests were substantial (K= 0.64). The results proved that the RTPCR assay could be a reliable and rapid alternative to VI assay for detection of AI viruses A H9 subtype H9 in fecal specimens.

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APA: Copy

NOUROUZIAN, H., & VASFI MARANDI, M.. (2007). DETECTION OF AVIAN INFLUENZA VIRUS OF H9 SUBTYPE IN THE FECES OF EXPERIMENTALLY INFECTED CHICKENS BY RT-PCR. ARCHIVES OF RAZI INSTITUTE, 62(4), 181-189. SID. https://sid.ir/paper/120026/en

Vancouver: Copy

NOUROUZIAN H., VASFI MARANDI M.. DETECTION OF AVIAN INFLUENZA VIRUS OF H9 SUBTYPE IN THE FECES OF EXPERIMENTALLY INFECTED CHICKENS BY RT-PCR. ARCHIVES OF RAZI INSTITUTE[Internet]. 2007;62(4):181-189. Available from: https://sid.ir/paper/120026/en

IEEE: Copy

H. NOUROUZIAN, and M. VASFI MARANDI, “DETECTION OF AVIAN INFLUENZA VIRUS OF H9 SUBTYPE IN THE FECES OF EXPERIMENTALLY INFECTED CHICKENS BY RT-PCR,” ARCHIVES OF RAZI INSTITUTE, vol. 62, no. 4, pp. 181–189, 2007, [Online]. Available: https://sid.ir/paper/120026/en

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