PCR-BASED DETECTION OF THEILERIA INFECTION AND MOLECULAR CHARACTERIZATION OF TAMS1 T. ANNULATA VACCINE STRAIN

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HABIBI GH.R.; ESMAEILNIA K.; BOZORGI S.; NAJAR E.; HASHEMI FESHARAKI R.; BORDBAR N.

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Journal: ARCHIVES OF RAZI INSTITUTE Year:2007 | Volume:62 | Issue:2 Page(s): 83-89

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Title

PCR-BASED DETECTION OF THEILERIA INFECTION AND MOLECULAR CHARACTERIZATION OF TAMS1 T. ANNULATA VACCINE STRAIN

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Keywords

THEILERIA ANNULATAQ3

PCRQ3

PHYLOGENETICQ4

Abstract

In order to develop a method for detecting and identification of THEILERIA ANNULATA, the specific primers from the major merozoite-piroplasm surface antigen sequence of Theileria (Tams1) were used to detect the T. anniulataby nested-PCR technique. The assay provides a valuable tool for the identification of THEILERIA ANNULATA directly from clinical samples and enables determination of the infecting species by a facile technique with high sensitivity and specificity power. The sensitivity of the PCR was determined up to 1.34 pg infected DNA, and specificity of the PCR was confirmed by DNA sequencing. The Tams1 nested-PCR assay will facilitate parasite infection follow up and might improve diagnosis and therapeutic approach of bovine tropical the ileriosis. Moreover, multiple alignment and PHYLOGENETIC analysis of Tams1 sequences of available strains/isolates showed that there is a particular restriction site in T. annulata Iran-vaccine strain could be recognized by AvaII enzyme. These findings can be used in further disease control and prevention program as wed as epidemiological studies.

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APA: Copy

HABIBI, GH.R., ESMAEILNIA, K., BOZORGI, S., NAJAR, E., HASHEMI FESHARAKI, R., & BORDBAR, N.. (2007). PCR-BASED DETECTION OF THEILERIA INFECTION AND MOLECULAR CHARACTERIZATION OF TAMS1 T. ANNULATA VACCINE STRAIN. ARCHIVES OF RAZI INSTITUTE, 62(2), 83-89. SID. https://sid.ir/paper/120061/en

Vancouver: Copy

HABIBI GH.R., ESMAEILNIA K., BOZORGI S., NAJAR E., HASHEMI FESHARAKI R., BORDBAR N.. PCR-BASED DETECTION OF THEILERIA INFECTION AND MOLECULAR CHARACTERIZATION OF TAMS1 T. ANNULATA VACCINE STRAIN. ARCHIVES OF RAZI INSTITUTE[Internet]. 2007;62(2):83-89. Available from: https://sid.ir/paper/120061/en

IEEE: Copy

GH.R. HABIBI, K. ESMAEILNIA, S. BOZORGI, E. NAJAR, R. HASHEMI FESHARAKI, and N. BORDBAR, “PCR-BASED DETECTION OF THEILERIA INFECTION AND MOLECULAR CHARACTERIZATION OF TAMS1 T. ANNULATA VACCINE STRAIN,” ARCHIVES OF RAZI INSTITUTE, vol. 62, no. 2, pp. 83–89, 2007, [Online]. Available: https://sid.ir/paper/120061/en

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