ISOLATION AND IDENTIFICATION OF MYCOPLASMA AGALACTIAE BY CULTURE AND POLYMERASE CHAIN REACTION IN SHEEP AND GOAT MILK SAMPLES IN KORDESTAN PROVINCE, IRAN

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MORADI BIDHENDI S.; KHAKI P.; PILEHCHIAN LANGROUDI R.

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Journal: ARCHIVES OF RAZI INSTITUTE Year:2011 | Volume:66 | Issue:1 Page(s): 11-16

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Information Journal Paper

Title

ISOLATION AND IDENTIFICATION OF MYCOPLASMA AGALACTIAE BY CULTURE AND POLYMERASE CHAIN REACTION IN SHEEP AND GOAT MILK SAMPLES IN KORDESTAN PROVINCE, IRAN

Author(s)

MORADI BIDHENDI S. | KHAKI P. | PILEHCHIAN LANGROUDI R. | Issue Writer Certificate

Keywords

MYCOPLASMA AGALACTIAEQ3

CULTUREQ3

PCRQ3

SHEEPQ3

GOATQ3

Abstract

Contagious agalactiae (C.A.) is one of the most common disease affecting small ruminants which is caused by MYCOPLASMA AGALACTIAE. This disease is particularly widespread around the world and Iran is one of the countries that C.A. is present. The aim of this study was isolation and identification of M. agalactiae (MG) with CULTURE and PCR technique in milk samples in Kordestan province, Iran. A total of 367 milk samples were collected from SHEEP and GOAT. Specific published primers amplify a 375 bp gene of MG were used for PCR. Twenty (5.5%) out of 367 were positive in PPLO agar and 5 (25%) out of these isolates were positive with MYCOPLASMA AGALACTIAE primers. Four (75%) out of 5 isolates was from SHEEP and 1 (25%) from GOAT. Result of PCR with 367 milk samples showed that 11 (3%) of them were positive with these primers. The isolation of M. agalactiae showed that C.A is present in Kordestan province and our results suggested that PCR method because of reduces the time consuming could be an alternative method beside CULTURE.

Cites

COMPARISON OF INFECTION RATE OF MYCOPLASMA AGALACTIAE IN AFFECTED SHEEP WITH GOATS TO CONTAGIOUS AGALACTIA IN KERMAN PROVINCE

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APA: Copy

MORADI BIDHENDI, S., KHAKI, P., & PILEHCHIAN LANGROUDI, R.. (2011). ISOLATION AND IDENTIFICATION OF MYCOPLASMA AGALACTIAE BY CULTURE AND POLYMERASE CHAIN REACTION IN SHEEP AND GOAT MILK SAMPLES IN KORDESTAN PROVINCE, IRAN. ARCHIVES OF RAZI INSTITUTE, 66(1), 11-16. SID. https://sid.ir/paper/120072/en

Vancouver: Copy

MORADI BIDHENDI S., KHAKI P., PILEHCHIAN LANGROUDI R.. ISOLATION AND IDENTIFICATION OF MYCOPLASMA AGALACTIAE BY CULTURE AND POLYMERASE CHAIN REACTION IN SHEEP AND GOAT MILK SAMPLES IN KORDESTAN PROVINCE, IRAN. ARCHIVES OF RAZI INSTITUTE[Internet]. 2011;66(1):11-16. Available from: https://sid.ir/paper/120072/en

IEEE: Copy

S. MORADI BIDHENDI, P. KHAKI, and R. PILEHCHIAN LANGROUDI, “ISOLATION AND IDENTIFICATION OF MYCOPLASMA AGALACTIAE BY CULTURE AND POLYMERASE CHAIN REACTION IN SHEEP AND GOAT MILK SAMPLES IN KORDESTAN PROVINCE, IRAN,” ARCHIVES OF RAZI INSTITUTE, vol. 66, no. 1, pp. 11–16, 2011, [Online]. Available: https://sid.ir/paper/120072/en

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