SHORT COMMUNICATION: SEROLOGICAL EVALUATION OF EXPERIMENTAL TOXOPLASMA GONDII INFECTION IN CATS BY USING IMMUNOBLOTTING BASED ON AN AFFINITY PURIFIED SURFACE ANTIGEN

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HOSSEININEJAD M.

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Journal: ARCHIVES OF RAZI INSTITUTE Year:2012 | Volume:67 | Issue:2 Page(s): 161-166

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Title

SHORT COMMUNICATION: SEROLOGICAL EVALUATION OF EXPERIMENTAL TOXOPLASMA GONDII INFECTION IN CATS BY USING IMMUNOBLOTTING BASED ON AN AFFINITY PURIFIED SURFACE ANTIGEN

Author(s)

HOSSEININEJAD M. | Issue Writer Certificate

Keywords

CATSQ3

IMMUNOBLOTQ3

P30Q3

SAG1Q3

TOXOPLASMA GONDIIQ3

Abstract

Toxoplasma gondii is an apicomplexan parasite that infects human and almost all warm-blooded animals. The life cycle of the parasite includes an asexual reproduction in intermediate hosts (Mammals and birds) and a sexual reproduction in definitive hosts (Felidae). CATS are both the intermediate and the definitive host for T. gondii. The aim of this study was to investigate anti-T. gondii antibodies in CATS by using an IMMUNOBLOT method based on a major surface antigen; SAG1. Six sero-negative kittens were infected intra-peritoneally by Rh strain of T. gondii. Serum samples were collected and evaluated for the presence of IgG antibodies by using two techniques; IMMUNOBLOTting and indirect fluorescent antibody test (IFAT). SAG1 based IMMUNOBLOTting was able to detect anti-T. gondii antibodies at least eight days after infection. Comparative evaluation showed that this method is as sensitive as IFAT to diagnose T. gondii infection in CATS.

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APA: Copy

HOSSEININEJAD, M.. (2012). SHORT COMMUNICATION: SEROLOGICAL EVALUATION OF EXPERIMENTAL TOXOPLASMA GONDII INFECTION IN CATS BY USING IMMUNOBLOTTING BASED ON AN AFFINITY PURIFIED SURFACE ANTIGEN. ARCHIVES OF RAZI INSTITUTE, 67(2), 161-166. SID. https://sid.ir/paper/120107/en

Vancouver: Copy

HOSSEININEJAD M.. SHORT COMMUNICATION: SEROLOGICAL EVALUATION OF EXPERIMENTAL TOXOPLASMA GONDII INFECTION IN CATS BY USING IMMUNOBLOTTING BASED ON AN AFFINITY PURIFIED SURFACE ANTIGEN. ARCHIVES OF RAZI INSTITUTE[Internet]. 2012;67(2):161-166. Available from: https://sid.ir/paper/120107/en

IEEE: Copy

M. HOSSEININEJAD, “SHORT COMMUNICATION: SEROLOGICAL EVALUATION OF EXPERIMENTAL TOXOPLASMA GONDII INFECTION IN CATS BY USING IMMUNOBLOTTING BASED ON AN AFFINITY PURIFIED SURFACE ANTIGEN,” ARCHIVES OF RAZI INSTITUTE, vol. 67, no. 2, pp. 161–166, 2012, [Online]. Available: https://sid.ir/paper/120107/en

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