EVALUATION AND COMPARISON OF HELA, HEP2C AND VERO CELL LINES SENSITIVITY TO POLIO VACCINAL VIRUS USING MICRO AND MACRO VACCINE POTENCY TESTS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SOLEYMANI S.; ABEDI KIASARI B.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARCHIVES OF RAZI INSTITUTE Year:2012 | Volume:67 | Issue:2 Page(s): 125-131

Download Full-Text

Persian Verion

View:

1,166

Download:

632

Cites:

Information Journal Paper

Title

EVALUATION AND COMPARISON OF HELA, HEP2C AND VERO CELL LINES SENSITIVITY TO POLIO VACCINAL VIRUS USING MICRO AND MACRO VACCINE POTENCY TESTS

Author(s)

SOLEYMANI S. | ABEDI KIASARI B. | Issue Writer Certificate

Keywords

POLIO VACCINEQ3

POTENCY TESTQ3

HELAQ3

HEP2CQ3

VEROQ3

Abstract

Poliomyelitis, an acute viral infectious disease caused by poliovirus, still remains a public health problem in developing countries. Despite the global effort to eradicate polio, continuing the polio immunization with a potent and safe vaccine is essential. For accurate vaccine evaluation, three types of cell lines including HELA, HEP2C and VERO were evaluated and compared using two methods of POLIO VACCINE POTENCY TESTs (micro & macro). For cells comparison, five different batches from POLIO VACCINEs were tested and to develop the test, five variables including viruses, cells, serum, media and Co2 were studied. For validation, the titer of which has been well established as a working reference preparation (WRP) was applied to control the accuracy and reproducibility of the testing system. Multiple comparisons were performed by analysis of variance (ANOVA) followed by Tokey HDS and LSD. No significant differences were found between the potency of vaccine batches and between macro and micro methods. Reduction in cells sensitivity and potency of vaccines was found with increasing passage number. Significant differences were found between the sensitivity of the cell lines. The highest potency of POLIO VACCINEs was obtained using HELA cells (GMT in macro and micro test =10 6.35); HEP2C cells were afterwards (GMT in macro=10 6.01 and in micro test=10 5.94); VERO cells were lowest (GMT in macro=10 5.78 and in micro test=10 5.72). So, the sensitivity and accuracy of the POTENCY TEST for evaluation of the POLIO VACCINE in immunization program in Iran will be assured using the HELA cell line with low passage number in macro and micro methods.

Cites

No record.

References

No record.

Cite

APA: Copy

SOLEYMANI, S., & ABEDI KIASARI, B.. (2012). EVALUATION AND COMPARISON OF HELA, HEP2C AND VERO CELL LINES SENSITIVITY TO POLIO VACCINAL VIRUS USING MICRO AND MACRO VACCINE POTENCY TESTS. ARCHIVES OF RAZI INSTITUTE, 67(2), 125-131. SID. https://sid.ir/paper/120110/en

Vancouver: Copy

SOLEYMANI S., ABEDI KIASARI B.. EVALUATION AND COMPARISON OF HELA, HEP2C AND VERO CELL LINES SENSITIVITY TO POLIO VACCINAL VIRUS USING MICRO AND MACRO VACCINE POTENCY TESTS. ARCHIVES OF RAZI INSTITUTE[Internet]. 2012;67(2):125-131. Available from: https://sid.ir/paper/120110/en

IEEE: Copy

S. SOLEYMANI, and B. ABEDI KIASARI, “EVALUATION AND COMPARISON OF HELA, HEP2C AND VERO CELL LINES SENSITIVITY TO POLIO VACCINAL VIRUS USING MICRO AND MACRO VACCINE POTENCY TESTS,” ARCHIVES OF RAZI INSTITUTE, vol. 67, no. 2, pp. 125–131, 2012, [Online]. Available: https://sid.ir/paper/120110/en

Related Journal Papers