CLASSICAL METHODS AND SPECIFIC PRIMERS IN
DETECTION OF ENDOPHYTIC FUNGI IN SOME
GRAMINEUOS PLANTS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GANJALI R.; SHARIF NABI B.; MIRLOUHI A.F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Rostaniha Year:2004 | Volume:5 | Issue:1 Page(s): 37-51

Download Full-Text

Persian Verion

View:

1,282

Download:

Cites:

Information Journal Paper

Title

CLASSICAL METHODS AND SPECIFIC PRIMERS IN DETECTION OF ENDOPHYTIC FUNGI IN SOME GRAMINEUOS PLANTS

Author(s)

GANJALI R. | SHARIF NABI B. | MIRLOUHI A.F. | Issue Writer Certificate

Keywords

ENDOPHYTESQ4

SPECIFIC PRIMERSQ4

GRAMINEOUS PLANTSQ4

MORPHOLOGICAL CHARACTERSQ2

Abstract

Mutual relationship between endophytic fungi and some gramineuos plants is one of the recently known host-microbe interactions. Endophytic fungi are classified in class Ascomycetes, order Hypocreales, tribe Balansieae and mostly genus Neotyphodium. They are transferred into the progeny via maternal stock and have many beneficial effects to their host plants, such as resistance to pests and diseases, environmental stresses such as pH fluctuation, drought, salinity and grazing because Iran has a wide range of plant genomic resources and is known as one of the origins of GRAMINEOUS PLANTS, it is necessary to know more about endophytic fungi and their presence by using classical and molecular methods. Twenty isolates of endophytic fungi were obtained from seed and leaf sheaths of Bromus tomentellus, Lolium prenne, Festuca arundinacea and Festuca pratensis. Mycelia of endophytic fungi were observed red or pink in seed and leaf sheath when stained by Rose Bengal. The DNA of endophytic fungi was. extracted by Murry and Thompson method with minor modification. In order to confirm the identity of ENDOPHYTES, three universal and specific primer e. g. ITS1, ITS4, IS1, IS3, and 11-1, 11-2 were used. All isolates showed a 550-750 bp band with ITS1, ITS4 and eighteen of them showed a 444 bp band with IS1, IS3 primers, which is an indicator of Neotyphodium ENDOPHYTES. When primers 11-1,11-2 were used, a 1000bp band was not observed in all isolates. Isolates FaSm and FpGon3 that did not shown a 444 bp band, may probably differ from Neotyphodium endophytic fungi.

Cites

تاثیر قارچ های اندوفیت گیاهان فسکیوی بلند و مرتعی در کنترل بیولوژیک شپشک مومی ریشه Phenococcus solani Ferris (Hom.:Pseudococcidae)

References

No record.

Cite

APA: Copy

GANJALI, R., SHARIF NABI, B., & MIRLOUHI, A.F.. (2004). CLASSICAL METHODS AND SPECIFIC PRIMERS IN DETECTION OF ENDOPHYTIC FUNGI IN SOME GRAMINEUOS PLANTS. ROSTANIHA, 5(1), 37-51. SID. https://sid.ir/paper/1283/en

Vancouver: Copy

GANJALI R., SHARIF NABI B., MIRLOUHI A.F.. CLASSICAL METHODS AND SPECIFIC PRIMERS IN DETECTION OF ENDOPHYTIC FUNGI IN SOME GRAMINEUOS PLANTS. ROSTANIHA[Internet]. 2004;5(1):37-51. Available from: https://sid.ir/paper/1283/en

IEEE: Copy

R. GANJALI, B. SHARIF NABI, and A.F. MIRLOUHI, “CLASSICAL METHODS AND SPECIFIC PRIMERS IN DETECTION OF ENDOPHYTIC FUNGI IN SOME GRAMINEUOS PLANTS,” ROSTANIHA, vol. 5, no. 1, pp. 37–51, 2004, [Online]. Available: https://sid.ir/paper/1283/en

Related Journal Papers