EFFECTS OF STERILIZATION PROTOCOL AND PRE-CHILLING TREATMENT ONIN VITRO SEED GERMINATION OFLEVISTICUM OFFICINALE KOCH

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KHATIBZADEH R.; AZIZI M.; AROUIEE H.; FARSI M.

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Journal: Journal of Horticultural Science Year:2013 | Volume:27 | Issue:2 Page(s): 130-138

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Title

EFFECTS OF STERILIZATION PROTOCOL AND PRE-CHILLING TREATMENT ONIN VITRO SEED GERMINATION OFLEVISTICUM OFFICINALE KOCH

Author(s)

KHATIBZADEH R. | AZIZI M. | AROUIEE H. | FARSI M. | Issue Writer Certificate

Keywords

LEVISTICUM OFFICINALEQ2

MEDICINAL PLANTSQ2

SEED GERMINATIONQ3

IN VITRO CULTUREQ2

Abstract

To protect and multiply important and rare plant resources, IN VITRO CULTURE serves as a more efficient alternative to traditional propagation approaches.LEVISTICUM OFFICINALE Koch. a member of Apiaceae is an important, endangered and neglected species in Iran, which has been shown to have diuretic, spasmolytic and carminative effects.In order to supply enough plant materials for micro-propagation of this herb and study effects of different methods of disinfection and stratification onin vitro SEED GERMINATION, a factorial experiment laid out in a completely randomized design was set out to establish sterile plants out of seed culture. It was concluded that a pre-chilling treatment for 3 months resulted in maximum percent of germination (92%) and the largest germination rate.The best superficial sterilization protocol was proofed to be soaking in 70% (v: v) ethanol for 30 s and then, using of 2% (v: v) dilution of NaOCl for 15 min, followed by 3 rinses in sterile distilled water.

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APA: Copy

KHATIBZADEH, R., AZIZI, M., AROUIEE, H., & FARSI, M.. (2013). EFFECTS OF STERILIZATION PROTOCOL AND PRE-CHILLING TREATMENT ONIN VITRO SEED GERMINATION OFLEVISTICUM OFFICINALE KOCH. JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY), 27(2), 130-138. SID. https://sid.ir/paper/142243/en

Vancouver: Copy

KHATIBZADEH R., AZIZI M., AROUIEE H., FARSI M.. EFFECTS OF STERILIZATION PROTOCOL AND PRE-CHILLING TREATMENT ONIN VITRO SEED GERMINATION OFLEVISTICUM OFFICINALE KOCH. JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY)[Internet]. 2013;27(2):130-138. Available from: https://sid.ir/paper/142243/en

IEEE: Copy

R. KHATIBZADEH, M. AZIZI, H. AROUIEE, and M. FARSI, “EFFECTS OF STERILIZATION PROTOCOL AND PRE-CHILLING TREATMENT ONIN VITRO SEED GERMINATION OFLEVISTICUM OFFICINALE KOCH,” JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY), vol. 27, no. 2, pp. 130–138, 2013, [Online]. Available: https://sid.ir/paper/142243/en

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