The Role of Ecotype, Explant and Plant Growth Regulators on Cell Suspension Culture of Ferulago angulata (Schlecht. ) Boiss.

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

alavi seyed mehran; MASOUMIASL ASAD; ZARE NASER; ASGHARI ZAKARIA RASOOL; Sheikhzadeh Mosaddegh Parisa

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Horticultural Science Year:2019 | Volume:33 | Issue:3 Page(s): 525-536

Download Full-Text

Persian Verion

View:

546

Download:

Cites:

Information Journal Paper

Title

The Role of Ecotype, Explant and Plant Growth Regulators on Cell Suspension Culture of Ferulago angulata (Schlecht. ) Boiss.

Author(s)

Keywords

CallusQ2

GenotypeQ1

PCVQ2

Plant growth regulatorQ1

SCVQ1

Abstract

Introduction: The main habitat of Chavil, Ferulago angulata, in Iran is Zagros area. This plant has a rejuvenating effect and is used to treat digestive diseases and intestinal worms. Because the different explants show different amounts of callogenesis under the effect of different growth regulators, selection of an optimal explant and suitable Plant growth regulators combination has a significant effect on the production of Callus and their suspension culture. There is no reports on Ferulago angulata callogenesis and its cell suspension culture. Therefore, this study was designed and implemented to optimize Callus production and cell suspension culture in this important medicinal plant. Materials and Methods: Seeds of Chavil were collected from four different habitats in Kohgilooyeh and BoyerAhmad Province in Southwest of Iran include Abenahr, Guayoune, Vezg and Sisakhat. Seedlings obtained from embryo culture were used to prepare the explants. Various explants (leaf, root and stem) were cultured on MS medium supplemented with different concentrations (0, 0. 5, 1 and 2 mgl-1) of NAA and BAP. Callus traits were evaluated and from the best culture medium, the best explants and the best PGRs composition for callogenesis of each ecotype were used to cell suspension culture. In order to study the growth rate of cells in suspension culture and plotting the curve of cells growth, two cell density indices and packed cell volume index were evaluated. To determine the cell density index, every 3 days, 10 ml of cell suspension were transferred to the graded falcon and centrifuged at 5000 g for 5 minutes, and the percentage of sediment cells was calculated as the total volume. To determine the packed cell volume index, also every 3 days, 10 ml of culture medium containing cells were transferred to the graded falcon and stored for 30 minutes to precipitate cells and cell masses. Finally, the cell volume was recorded and was calculated as percentage of the total collected medium. Results and Discussion: According to the callogenesis percentage, the best ecotype is Abenahr and best explant is leaf explant. The highest level of NAA is 2 mgl-1, and the best level of BAP is 2 mgl-1, which causes 100 callogenesis percentage. The best medium for cell suspension culture is MS medium containing 2 mgl-1 NAA and 0. 5 mgl-1 BAP for Callus was obtained from leaf explant of Abenahr ecotype. Along with these Plant growth regulators, 2, 4-D was used in combination with BAP to form suspension culture. The results also showed that 2 mgl-1 2, 4-D plus 0. 5 mgl-1 BAP were useful in producing suspensions. The difference between 2, 4-D +BAP and NAA + BAP combinations more cell volumes were observed, and cell suspension was created at a faster rate and in less time, which is an advantage in research work. Growth rate of cell suspension originated from the leaf explant was higher than root explant. In terms of culturing cell suspension, the Abenahr ecotype was favorable compared to other ecotypes. During cell suppression culture of Cyperus aromaticus by applying different levels of NAA, cell growth was increased up to 3 weeks after application, and then decreased. By applying 2, 4-D, cell growth also increased until the third week, and after the third week, cell growth declined, which was very low growth rate compared with the NAA. In cell suspension culture of sugar beet, using 2, 4-D was much more effective than NAA on all explants. In the present study, 2, 4-D was also more effective than NAA for cell suspension culture of Chavil. Conclusion: In general, the Abenahr was the best ecotype among of investigated. The explants in both Callus culture and the suspension culture, and the best combination of Plant growth regulator in both culture was 2 mgl-1 NAA plus 0. 5 mgl-1 BAP.

Cites

No record.

References

No record.

Cite

APA: Copy

alavi, seyed mehran, MASOUMIASL, ASAD, ZARE, NASER, ASGHARI ZAKARIA, RASOOL, & Sheikhzadeh Mosaddegh, Parisa. (2019). The Role of Ecotype, Explant and Plant Growth Regulators on Cell Suspension Culture of Ferulago angulata (Schlecht. ) Boiss.. JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY), 33(3 ), 525-536. SID. https://sid.ir/paper/142448/en

Vancouver: Copy

alavi seyed mehran, MASOUMIASL ASAD, ZARE NASER, ASGHARI ZAKARIA RASOOL, Sheikhzadeh Mosaddegh Parisa. The Role of Ecotype, Explant and Plant Growth Regulators on Cell Suspension Culture of Ferulago angulata (Schlecht. ) Boiss.. JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY)[Internet]. 2019;33(3 ):525-536. Available from: https://sid.ir/paper/142448/en

IEEE: Copy

seyed mehran alavi, ASAD MASOUMIASL, NASER ZARE, RASOOL ASGHARI ZAKARIA, and Parisa Sheikhzadeh Mosaddegh, “The Role of Ecotype, Explant and Plant Growth Regulators on Cell Suspension Culture of Ferulago angulata (Schlecht. ) Boiss.,” JOURNAL OF HORTICULTURE SCIENCE (AGRICULTURAL SCIENCES AND TECHNOLOGY), vol. 33, no. 3 , pp. 525–536, 2019, [Online]. Available: https://sid.ir/paper/142448/en

Related Journal Papers