Using Nested-PCR in the Study of Cryptosporidium spp Parasite Spread in Domestic Bird

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Abdullah Al Kaeebi Saafa Ressan; Saleh Al Difaie Rana; Ibrahim Mohammad Fatima

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Journal Paper

Paper Information

Journal: Agricultural Biotechnology Journal Year:2024 | Volume:16 | Issue:2 Page(s): 281-295

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Information Journal Paper

Title

Using Nested-PCR in the Study of Cryptosporidium spp Parasite Spread in Domestic Bird

Author(s)

Abdullah Al Kaeebi Saafa Ressan | Saleh Al Difaie Rana | Ibrahim Mohammad Fatima | Issue Writer Certificate

Keywords

Nested-PCRQ4

Microscopic ExaminationQ4

Infection RateQ4

birdsQ4

and CryptosporidiumQ4

Abstract

ObjectivesThis study examined the number of cases of Cryptosporidium spp. infection in domestic birds in Diwaniyah Governorate, as well as the consequences of this illness and the methods used to diagnose it. Materials and MethodsIn the study, 205 samples from different bird species were analyzed without gender being taken into account. Some of the methods used for diagnosis were the Acid Fast Stain method and the DNA nested Polymerization Technique (PCR). Nested - PCR proved out to be better than AFS because it could find Cryptosporidium faster, with highest accuracy, and sensitivity. ResultsThe results showed that 90 of the samples had Cryptosporidium infections. Among the bird species that were studied, falseco eleonorae had the lowest incidence of cryptosporidiosis at 16%, while gallus gallus domesticus had the highest incidence at 53.3%. Out of the 60 samples taken for each species, 48.3% of Meleagris gallopova and 41.6% of Anas platyrhynchos were infected. The microscope examination showed a 43.9% Infection Rate (90 out of 205 samples) when compared to the other method. To use Nested-PCR for molecular identification, DNA had to be taken from positive infected samples using Proteinase K method. This produced positive bands that were usually amplified at around 587 bp. The Nested-PCR test showed that 47.5% of the samples (19 out of 40) were infected. ConclusionsFinally, this study highlights the critical need for efficient diagnostic tools and intervention plans by demonstrating the very high incidence of Cryptosporidium infection in domestic birds in Diwaniyah Governorate. In order to prevent the spread of Cryptosporidiosis in birds, the results stress the use of nested polymerase chain reaction (PCR) methods for infection diagnosis.

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