INTRODUCING A NEW METHOD OF GENOMIC DNA EXTRACTION IN DICOTYLEDONOUS MEDICAL PLANTS

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HOSSEINPOUR AZAD N.; NEMATZADEH G.

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Journal: PLANT AND ECOSYSTEM Year:2012 | Volume:8 | Issue:31 Page(s): 111-120

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Information Journal Paper

Title

INTRODUCING A NEW METHOD OF GENOMIC DNA EXTRACTION IN DICOTYLEDONOUS MEDICAL PLANTS

Author(s)

HOSSEINPOUR AZAD N. | NEMATZADEH G. | Issue Writer Certificate

Keywords

ECHIUM AMOENUMQ2

GENETIC DIVERSITYQ2

DNA EXTRACTIONQ2

STEQ2

BSAQ2

Abstract

To provide DNA EXTRACTION buffer, various materials are used. Some of these materials are sensitive to temperature and others such as phenol-chloroform is dangerous for human health. Moreover, they needed liquid nitrogen and Rnase enzymes, many of the mentioned substances will be removed by using STE buffer. In previous methods, the researchers nedded substances such as BSA, which had high cost and sensivity to increasing of temperature while extraction process made it inevitable to use centrifuge device with cooler system. But most laboratories do not have these facilities. On the other hand, common methods need to employ solutions in high volumes, which altogether increase the cost of DNA EXTRACTION. For this reason, using common methods to extract DNA was not economical in the laboratories with fewer facilities. In optimized and proposed method, PVP was used instead of BSA in different experiments. in addition to having high resistance against temperature increasing, it removed secondary metabolites, specially poly phenols from cellular extracts of plant tissues, it also used this substance to reduce cell extract washing time with, component solutions of chloroform- isoamil alcohol to one stage. All the above mentioned factors cause this method to be applicable in a shorter time than common methods of DNA extracting. It also long with decreasing the component to less than a quarter of previous methods, the STE method was optimized as a simple, fast and cheap method to extract genomic DNA in a wide range of medicinal plants.

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References

GENETIC DIVERSITY IN SEVERAL ECHIUM AMOENUM FISCH & MEY. ECOTYPES FROM WESTERN AND NORTHERN REGIONS OF IRAN VIA RAPD MARKERS

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APA: Copy

HOSSEINPOUR AZAD, N., & NEMATZADEH, G.. (2012). INTRODUCING A NEW METHOD OF GENOMIC DNA EXTRACTION IN DICOTYLEDONOUS MEDICAL PLANTS. PLANT AND ECOSYSTEM, 8(31), 111-120. SID. https://sid.ir/paper/145184/en

Vancouver: Copy

HOSSEINPOUR AZAD N., NEMATZADEH G.. INTRODUCING A NEW METHOD OF GENOMIC DNA EXTRACTION IN DICOTYLEDONOUS MEDICAL PLANTS. PLANT AND ECOSYSTEM[Internet]. 2012;8(31):111-120. Available from: https://sid.ir/paper/145184/en

IEEE: Copy

N. HOSSEINPOUR AZAD, and G. NEMATZADEH, “INTRODUCING A NEW METHOD OF GENOMIC DNA EXTRACTION IN DICOTYLEDONOUS MEDICAL PLANTS,” PLANT AND ECOSYSTEM, vol. 8, no. 31, pp. 111–120, 2012, [Online]. Available: https://sid.ir/paper/145184/en

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