INDUCTION OF EMBRYOGENESIS IN MICROSPORES OF TOMATO (LYCOPORSICUM ESCULONTUM MILL) CV. MICROTOM

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ENAYATI SHARIAT PANAHI M.; TOURAEV A.; HERBELE BROS E.

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Journal: SEED AND PLANT PRODUCTION JOURNAL Year:2009 | Volume:25-2 | Issue:3 Page(s): 317-330

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Title

INDUCTION OF EMBRYOGENESIS IN MICROSPORES OF TOMATO (LYCOPORSICUM ESCULONTUM MILL) CV. MICROTOM

Author(s)

ENAYATI SHARIAT PANAHI M. | TOURAEV A. | HERBELE BROS E. | Issue Writer Certificate

Keywords

TOMATOQ3

MICROSPORE CULTUREQ3

EMBRYOGENESISQ2

COLCHICINE AND HAPLOIDQ2

Abstract

Haploid breeding is the most efficient method for the production of pure lines. In this research, TOMATO MICROSPORE CULTURE method was studied to develop an EMBRYOGENESIS protocol. The influence of several stresses, i.e. low and high temperatures, starvation and colchicine applied on isolated TOMATO microspores on the induction of microspore EMBRYOGENESIS was investigated. Microspores in different stages of development were isolated from surface-sterilized flower buds and cultured in medium AT3 or starvation medium B, with or without colchicine, at different temperatures for varying lengths of time. After the stress treatments, microspores were transferred to modified AT3 medium supplemented with phytohormones and incubated at 25oC in the dark. The results showed that the late-uninucleate stage microspore, B medium and magnetic stirring were the best developmental stage, medium and method, respectively for isolating viable microspores. Treatment with colchicine (25 mM) in combination with low temperature at 4oC for 72h also induced more than 35% of the cultured TOMATO microspores to divide symmetrically forming calli and embryoids.

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APA: Copy

ENAYATI SHARIAT PANAHI, M., TOURAEV, A., & HERBELE BROS, E.. (2009). INDUCTION OF EMBRYOGENESIS IN MICROSPORES OF TOMATO (LYCOPORSICUM ESCULONTUM MILL) CV. MICROTOM. SEED AND PLANT PRODUCTION JOURNAL, 25-2(3), 317-330. SID. https://sid.ir/paper/147112/en

Vancouver: Copy

ENAYATI SHARIAT PANAHI M., TOURAEV A., HERBELE BROS E.. INDUCTION OF EMBRYOGENESIS IN MICROSPORES OF TOMATO (LYCOPORSICUM ESCULONTUM MILL) CV. MICROTOM. SEED AND PLANT PRODUCTION JOURNAL[Internet]. 2009;25-2(3):317-330. Available from: https://sid.ir/paper/147112/en

IEEE: Copy

M. ENAYATI SHARIAT PANAHI, A. TOURAEV, and E. HERBELE BROS, “INDUCTION OF EMBRYOGENESIS IN MICROSPORES OF TOMATO (LYCOPORSICUM ESCULONTUM MILL) CV. MICROTOM,” SEED AND PLANT PRODUCTION JOURNAL, vol. 25-2, no. 3, pp. 317–330, 2009, [Online]. Available: https://sid.ir/paper/147112/en

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