ASSESSMENT OF GENETIC DIVERSITY AMONG IRANIAN PISTACHIOS USING MICROSATELLITES ISOLATED FROM PISTACIA KHINJUK

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ARABNEZHAD H.; BAHAR MASOUD; TAJABADIPOUR A.

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Journal: Journal of Water and Soil Science Year:2008 | Volume:12 | Issue:45 (A) Page(s): 207-217

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Title

ASSESSMENT OF GENETIC DIVERSITY AMONG IRANIAN PISTACHIOS USING MICROSATELLITES ISOLATED FROM PISTACIA KHINJUK

Author(s)

ARABNEZHAD H. | BAHAR MASOUD | TAJABADIPOUR A. | Issue Writer Certificate

Keywords

PISTACHIOQ3

P. KHINJUKQ3

GENETIC DIVERSITYQ3

SSR MARKERQ3

Abstract

Microsatellite DNA markers isolated from wild species khinjuk (Pistacia khinjuk Stocks.) was used to evaluate the GENETIC DIVERSITY available in Iranian PISTACHIO cultivars. Out of the 27 SSR primers tested initially, 25 could amplify the DNA in different PISTACHIO cultivars, of which 19 primer pairs produced clear bands. Based on the amplification profiles of the genotypes by the remaining primer pairs, eight primers produced a monomorphic product and other 11 microsatellites markers were found polymorphic among the genotypes. The number of putative alleles amplified by each polymorphic SSR locus ranged from two to eleven alleles with a total of 48 alleles. An average of alleles and observed heterozygosity per locus was 3.69 and 0.69 respectively, showing that these microsatellites are highly informative for PISTACHIO fingerprinting. The UPGMA cluster plots based on nei index placed the 20 commercial PISTACHIO cultivars into a major group containing three distinguished subgroups; however, genotypes, namely, Ghazvini zudras and Sarakhs (wild P. vera), were clearly situated into two distinct clusters, distant from the domesticated genotypes studied here. Both Ghazvini zudras and Sarakhs are known as small-fruited genotypes which are grown in restricted regions. Therefore, the distinctness of these genotypes can be attributed to their geographical isolation and morphological characteristics. It seems that Ghazvini zudras probably originated from Sarakhs variety which posses an important role in development of PISTACHIO cultivars. The present study revealed that the khinjuk PISTACHIO microsatellites are well distributed in the genome of P.vera, and are informative for estimating the extent of GENETIC DIVERSITY and characterization of PISTACHIO cultivars.

Cites

STUDY OF GENETIC DIVERSITY IN IRANIAN PISTACHIO CULTIVARS WITH INTER- MICROSATELLITE ISSR MARKERS

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APA: Copy

ARABNEZHAD, H., BAHAR, MASOUD, & TAJABADIPOUR, A.. (2008). ASSESSMENT OF GENETIC DIVERSITY AMONG IRANIAN PISTACHIOS USING MICROSATELLITES ISOLATED FROM PISTACIA KHINJUK. WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES), 12(45 (A)), 207-217. SID. https://sid.ir/paper/14787/en

Vancouver: Copy

ARABNEZHAD H., BAHAR MASOUD, TAJABADIPOUR A.. ASSESSMENT OF GENETIC DIVERSITY AMONG IRANIAN PISTACHIOS USING MICROSATELLITES ISOLATED FROM PISTACIA KHINJUK. WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES)[Internet]. 2008;12(45 (A)):207-217. Available from: https://sid.ir/paper/14787/en

IEEE: Copy

H. ARABNEZHAD, MASOUD BAHAR, and A. TAJABADIPOUR, “ASSESSMENT OF GENETIC DIVERSITY AMONG IRANIAN PISTACHIOS USING MICROSATELLITES ISOLATED FROM PISTACIA KHINJUK,” WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES), vol. 12, no. 45 (A), pp. 207–217, 2008, [Online]. Available: https://sid.ir/paper/14787/en

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