DESIGNING, CONSTRUCTION AND TRANSFORMATION OF SILENCING CONSTRUCTS FOR F3’H AND F3’5’H GENES IN GRAPEVINE CV. SHIRAZ

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PEJMAN MEHR MARYAM; EBADI ALI; MOUSAVI AMIR; MCDAVID DEBRA; WALKER AMANDA R.

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Journal: Iranian Journal of Horticultural Science Year:2015 | Volume:46 | Issue:1 Page(s): 1-16

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Title

DESIGNING, CONSTRUCTION AND TRANSFORMATION OF SILENCING CONSTRUCTS FOR F3’H AND F3’5’H GENES IN GRAPEVINE CV. SHIRAZ

Author(s)

Keywords

VITIS VINIFERAQ2

GENE CONSTRUCTQ2

SILENCINGQ1

F3’HQ2

F3’5’HQ2

Abstract

In order to study F3’5’H and F3’H function and their intraction in the grapevine flavonoids biosynthetic pathway, designing and constraction of suitable constructs encoding ihpRNA was performed and transgenic lines containing SILENCING construct of F3’H and simultaneous SILENCING construct of F3’5’H and F3’H were created. For this purpose, fragments were selected from both genes and inserted into pHANNIBAL vector as inverted repeats. Using binary vector system, each construct inserted into p27 mod GFP 4a vector and were used for Agrobacterium transformation. Embryogenic calli, drived from anther culture, were co-cultivated with Agrobacterium for transformation. Expression of GFP gene was detected in transgenic embryogenic calli, 5 days after inoculation using microscope. Finally, 42 and 34 lines were generated from single (F3’H) and simultaneous SILENCING (F3’5’H and F3’H) cultures, respectively. PCR analysis confirmed 36 single (F3’H) SILENCING line and 27 simultaneous SILENCING (F3’5’H and F3’H) lines, showing high efficacy of regeneration and transformation methods.

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APA: Copy

PEJMAN MEHR, MARYAM, EBADI, ALI, MOUSAVI, AMIR, MCDAVID, DEBRA, & WALKER, AMANDA R.. (2015). DESIGNING, CONSTRUCTION AND TRANSFORMATION OF SILENCING CONSTRUCTS FOR F3’H AND F3’5’H GENES IN GRAPEVINE CV. SHIRAZ. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 46(1), 1-16. SID. https://sid.ir/paper/148126/en

Vancouver: Copy

PEJMAN MEHR MARYAM, EBADI ALI, MOUSAVI AMIR, MCDAVID DEBRA, WALKER AMANDA R.. DESIGNING, CONSTRUCTION AND TRANSFORMATION OF SILENCING CONSTRUCTS FOR F3’H AND F3’5’H GENES IN GRAPEVINE CV. SHIRAZ. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2015;46(1):1-16. Available from: https://sid.ir/paper/148126/en

IEEE: Copy

MARYAM PEJMAN MEHR, ALI EBADI, AMIR MOUSAVI, DEBRA MCDAVID, and AMANDA R. WALKER, “DESIGNING, CONSTRUCTION AND TRANSFORMATION OF SILENCING CONSTRUCTS FOR F3’H AND F3’5’H GENES IN GRAPEVINE CV. SHIRAZ,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 46, no. 1, pp. 1–16, 2015, [Online]. Available: https://sid.ir/paper/148126/en

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