Effect of Salicylic Asid and Methyle Jasmonat Elicitors on Hypericin production in stJohn’ s wort (Hypericum perforatum L. ) cv. Topas Callus culture

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ZAMANI MOHAMMAD; MORADI HOSEIN; CHALAVI VIDA; KAZEMITABAR SEYED KAMAL

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Journal: Iranian Journal of Horticultural Science Year:2019 | Volume:49 | Issue:4 Page(s): 915-923

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Title

Effect of Salicylic Asid and Methyle Jasmonat Elicitors on Hypericin production in stJohn’ s wort (Hypericum perforatum L. ) cv. Topas Callus culture

Author(s)

ZAMANI MOHAMMAD | MORADI HOSEIN | CHALAVI VIDA | KAZEMITABAR SEYED KAMAL | Issue Writer Certificate

Keywords

ElicitorQ1

NaphtodianthronesQ1

Secondary metabolitesQ1

StJohn’s WortQ1

Abstract

Effects of salicylic acid and methyl Jasmonate on Hypericin production in callus culture of Hypericum perforatum L. cv Topas were investigated. This factorial experiment was carried out in a completely randomized design with two factors including the four levels of salicylic acid (225, 150, 75, 0 μ M) and methyl Jasmonate in three levels (150, 75, 0 μ M) with three replications at the research lab Group of Sari University of Agricultural Sciences and natural resources, deprtment of Horticultural Science. Analysis of variance showed significant differences among treatments. Effect of treatments on total flavonoid Methyl Jasmonate effect was significant. Interaction effect was significant for Hypericin, flavonoids and anthocyanins. According to the results of this study in cell cultures of H. perforatum, low concentration (100 micromolar and lower) of both salicylic acid and methyl Jasmonate Elicitors were effective for production of Hypericin. The results of this experiment showed that the highest amount of Hypericin produced from 150 μ M salicylic acid (SA) and 225 μ M salicylic acid in combination with 75 μ M methyl Jasmonate (MJ) after 21 days.

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APA: Copy

ZAMANI, MOHAMMAD, MORADI, HOSEIN, CHALAVI, VIDA, & KAZEMITABAR, SEYED KAMAL. (2019). Effect of Salicylic Asid and Methyle Jasmonat Elicitors on Hypericin production in stJohn’ s wort (Hypericum perforatum L. ) cv. Topas Callus culture. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 49(4 ), 915-923. SID. https://sid.ir/paper/148348/en

Vancouver: Copy

ZAMANI MOHAMMAD, MORADI HOSEIN, CHALAVI VIDA, KAZEMITABAR SEYED KAMAL. Effect of Salicylic Asid and Methyle Jasmonat Elicitors on Hypericin production in stJohn’ s wort (Hypericum perforatum L. ) cv. Topas Callus culture. IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2019;49(4 ):915-923. Available from: https://sid.ir/paper/148348/en

IEEE: Copy

MOHAMMAD ZAMANI, HOSEIN MORADI, VIDA CHALAVI, and SEYED KAMAL KAZEMITABAR, “Effect of Salicylic Asid and Methyle Jasmonat Elicitors on Hypericin production in stJohn’ s wort (Hypericum perforatum L. ) cv. Topas Callus culture,” IRANIAN JOURNAL OF HORTICULTURAL SCIENCES (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 49, no. 4 , pp. 915–923, 2019, [Online]. Available: https://sid.ir/paper/148348/en

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