Molecular detection and survey of tomato big bud phytoplasma in Zanjan province

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Shokri Mahnaz; Jafary Hossein; Azimi Moghadam Mohammadraza

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: GENETIC ENGINEERING AND BIOSAFETY JOURNAL Year:2023 | Volume:12 | Issue:1 Page(s): 123-130

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Information Journal Paper

Title

Molecular detection and survey of tomato big bud phytoplasma in Zanjan province

Author(s)

Shokri Mahnaz | Jafary Hossein | Azimi Moghadam Mohammadraza | Issue Writer Certificate

Keywords

Phytoplasma

BigBud

tomato

Molecular detection

PCR

Abstract

tomato big bud is one of the most important diseases of tomato caused by Phytoplasma in Iran and all over the world. In infected tomato plants, leaves turn to purple and buds become large, swollen and green that fail to develop normally and do not set fruit. For detection of the causal pathogen, we collected some samples from leaves and swollen buds of infected tomato plants as well as from leaves of some weeds in tomato fields such as Orobanche purpurea, Reseda sp, Amaranthus sp. Xanthium strumarium. Total DNA from all collected samples was extracted. To survey the pathogen in the body of some insects that are known as vectors, samples of two species that appeared more frequently in the tomato fields (leafhoppers and whiteflies) were collected and the total DNA was extracted. Molecular detection of the big bud Phytoplasma in all samples was carried out by PCR using two specific primers (P1/P7). The results showed that in infected samples of the tomato plants, a DNA fragment of 1800 bp in size was multiplied while in the control plant no DNA fragment was observed when the PCR products were visualized in the gel electrophoresis. The same size specific DNA fragment was detected only in PCR reaction coming from leafhoppers and samples of the Orbanche plant parasite. This research showed that the pathogen can be transmitted by leafhoppers and Orbanche while it cannot be transmitted by seeds and whiteflies. This molecular approach could be an effective and fast method for detection and survey of Phytoplasma and other fastidious plant pathogens.

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