MOLECULAR COMPARISON OF IRANIAN POPULATIONS OF MELOIDOGYNE JAVANICA AND MELOIDOGYNE INCOGNITA WITH PCR-RFLP

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MAHDIKHANI MOGHADAM ESMAT; KHEYRI A.; MOHAMMADI M.

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Journal: Journal of Water and Soil Science Year:2007 | Volume:10 | Issue:4 (B) Page(s): 405-411

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Information Journal Paper

Title

MOLECULAR COMPARISON OF IRANIAN POPULATIONS OF MELOIDOGYNE JAVANICA AND MELOIDOGYNE INCOGNITA WITH PCR-RFLP

Author(s)

MAHDIKHANI MOGHADAM ESMAT | KHEYRI A. | MOHAMMADI M. | Issue Writer Certificate

Keywords

MITOCHONDRIAL DNAQ3

MELOIDOGYNEQ3

RESTRICTION ENZYMESQ3

PCR-RFLPQ3

IRANQ3

Abstract

To carry out this study, total DNA was extracted from eggs and from second stage juveniles of several populations of MELOIDOGYNE javanica and MELOIDOGYNE incognita, using phenol/chloroform method. Following mgextraction, DNA was electrophoreses on 1% agarose gel to determine its quality and quantity. A specific primer pair (C2F3/1108; 23 and 20 nucleotides, respectively) was used to discriminate M.javanica from M. incognita populations using polymerase chain reaction (PCR). Primer annealing sites were located in the 3 portion of mitochondrial gene encoding cytochrome oxidase subunit II (COII) and in the 16S rRNA gene. Following PCR amplification, electrophoresis of amplified DNA showed 1.7 kb fragment in populations of both species. Digestion of 1.7 kb amplified product with Hinfl restriction endonuclease resulted in the generation of two DNA fragments of 0.7 and 1.0 kb in M. javanica and three DNA fragments of 0.3, 0.4 and 1.0 kb in M. incognita. There were no differences in the digestion patterns among various populations of each species examined.

Cites

ISOLATION OF ROOT-KNOT NEMATODE, MELOIDOGYNE CRUCIANI IN CUCUMBER GREENHOUSES OF JIROFT AND ASSESSMENT GENETIC DIVERSITY OF ITS POPULATION USING RAPD-PCR MARKER

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APA: Copy

MAHDIKHANI MOGHADAM, ESMAT, KHEYRI, A., & MOHAMMADI, M.. (2007). MOLECULAR COMPARISON OF IRANIAN POPULATIONS OF MELOIDOGYNE JAVANICA AND MELOIDOGYNE INCOGNITA WITH PCR-RFLP. WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES), 10(4 (B)), 405-411. SID. https://sid.ir/paper/15100/en

Vancouver: Copy

MAHDIKHANI MOGHADAM ESMAT, KHEYRI A., MOHAMMADI M.. MOLECULAR COMPARISON OF IRANIAN POPULATIONS OF MELOIDOGYNE JAVANICA AND MELOIDOGYNE INCOGNITA WITH PCR-RFLP. WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES)[Internet]. 2007;10(4 (B)):405-411. Available from: https://sid.ir/paper/15100/en

IEEE: Copy

ESMAT MAHDIKHANI MOGHADAM, A. KHEYRI, and M. MOHAMMADI, “MOLECULAR COMPARISON OF IRANIAN POPULATIONS OF MELOIDOGYNE JAVANICA AND MELOIDOGYNE INCOGNITA WITH PCR-RFLP,” WATER AND SOIL SCIENCE (JOURNAL OF SCIENCE AND TECHNOLOGY OF AGRICULTURE AND NATURAL RESOURCES), vol. 10, no. 4 (B), pp. 405–411, 2007, [Online]. Available: https://sid.ir/paper/15100/en

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