Investigating Changes in the Composition of Active Substances and the Amount of Essential Oil of Thymus vulgaris (L. ) Affected by the Foliar Application of Humic Acid and Mycorrhizal Species

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Taheri Asghari Mehdi; Mohamadzadeh Hamid

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Journal Paper

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Journal: Iranian Journal of Field Crop Science Year:2024 | Volume:11 | Issue:4 Page(s): 117-129

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Title

Investigating Changes in the Composition of Active Substances and the Amount of Essential Oil of Thymus vulgaris (L. ) Affected by the Foliar Application of Humic Acid and Mycorrhizal Species

Author(s)

Taheri Asghari Mehdi | Mohamadzadeh Hamid | Issue Writer Certificate

Keywords

Carotenoid

Limonene

Linalool

Myrcene

Abstract

In order to investigate the effects of foliar application of humic acid and mycorrhizal fungi symbiosis on Thymus vulgaris (L. ), a factorial experiments was conducted based on a randomized complete block design (RCBD) with three replications at the field of the research farm of Islamic Azad University, Takestan Branch during 2020-2021 and 2021-2022 years. Experimental factors included three species of mycorrhiza fungi (control, Glomus mosseae, G. etunicatum, and G. intradices) and humic acid fertilizer levels included (control, 150, and 300 mg. lit-1 ). The results showed that the effect of mycorrhiza and humic acid on chlorophyll a and b was significant. The highest amount of Carotenoid was obtained from the application of 300 mg/liter of humic acid and G. mosseae, which showed a 256% increase compared to the control. The results showed that the highest amount of phenolic compounds (0. 87 mg g-1 fresh weight) was obtained from the application of 300 mg/liter of humic acid without inoculation but humic acid treatments did not show any significant difference with G. mosseae mycorrhiza treatment. In addition, the interaction effect of mycorrhiza and humic acid on essential oil yield Limonene and linalol essential oil compounds was significant at the level of 1%. The highest essential oil yield (92. 45 kg hectare-1 ) was obtained from the treatment of 150 mg of humic acid and the application of mycorrhiza G. mosseae, which showed a 254% increase compared to the control. The highest amount of Limonene and linalol was obtained from the application of 300 mg/liter of humic acid and G. mosseae mycorrhiza, which showed an increase of 250 and 280%, respectively, compared to the control. According to the obtained results, the use of mycorrhiza especially G. mosseae and humic acid with a concentration of 300 mg/l can have a positive effect on the composition of effective substances and cause an increase in their amount

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