THE ISOLATION OF SPECIFIC BACTERIOPHAGE ESCHERICHIA COLI O157: H7 FROM AGRICULTURAL SOIL

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REZATOFIGHI S.E.; KHALEQHIAN P.; TAJBAKHSH A.

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Journal: JOURNAL OF VETERINARY MICROBIOLOGY Year:2014 | Volume:2 | Issue:2 (27) Page(s): 91-97

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Title

THE ISOLATION OF SPECIFIC BACTERIOPHAGE ESCHERICHIA COLI O157: H7 FROM AGRICULTURAL SOIL

Author(s)

REZATOFIGHI S.E. | KHALEQHIAN P. | TAJBAKHSH A. | Issue Writer Certificate

Keywords

BACTERIOPHAGEQ2

ESCHERICHIA COLIQ2

O157: H7Q2

AGRICULTURAL SOILQ2

Abstract

BACTERIOPHAGE treatment offers a possible alternative to conventional antibiotic therapy against multidrug-resistant bacteria. Phage therapy has already been proven to be advantageous since it is more specific, accurate and potent than antibiotics. The aim of this study was the isolation of E. coli O157: H7 specific phages. To isolate the phage from the samples of soil, AGRICULTURAL SOIL enrichment with animal manure, and waste were investigated. Spot assay and soft agar overlay technique were used for the isolation of phage. Electron Microscope was used for observation of phage structure. Specificity of the phage isolated was investigated. E. coli O157 phage was isolated from AGRICULTURAL SOIL enrichment with animal manure. This phage produced clear plaques (0.2 to 0.3 mm diameter) on E. coli O157 but not on the other tested bacteria. The number of phage was calculated 3×104 PFU/ml. Phage virions were observed and confirmed by TEM. The virulent phage isolated in this study may be used for biocontrol of E. coli O157 in animals and fresh foods without compromising the viability of other normal flora or food quality.

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APA: Copy

KHALEQHIAN, P., REZATOFIGHI, S.E., & TAJBAKHSH, A.. (2014). THE ISOLATION OF SPECIFIC BACTERIOPHAGE ESCHERICHIA COLI O157: H7 FROM AGRICULTURAL SOIL. JOURNAL OF VETERINARY MICROBIOLOGY, 2(2 (27)), 91-97. SID. https://sid.ir/paper/152142/en

Vancouver: Copy

KHALEQHIAN P., REZATOFIGHI S.E., TAJBAKHSH A.. THE ISOLATION OF SPECIFIC BACTERIOPHAGE ESCHERICHIA COLI O157: H7 FROM AGRICULTURAL SOIL. JOURNAL OF VETERINARY MICROBIOLOGY[Internet]. 2014;2(2 (27)):91-97. Available from: https://sid.ir/paper/152142/en

IEEE: Copy

P. KHALEQHIAN, S.E. REZATOFIGHI, and A. TAJBAKHSH, “THE ISOLATION OF SPECIFIC BACTERIOPHAGE ESCHERICHIA COLI O157: H7 FROM AGRICULTURAL SOIL,” JOURNAL OF VETERINARY MICROBIOLOGY, vol. 2, no. 2 (27), pp. 91–97, 2014, [Online]. Available: https://sid.ir/paper/152142/en

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