ANALYSIS OF GENETIC AND CYTOGENETIC VARIATIONS BETWEEN ALFALFA (MEDICAGO SATIVA L.) GENOTYPES IN IRAN

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FARSHADFAR MOHSEN; BOLOORCHIAN FATEMEH; SAFARI HOOSHMAND; SHIRVANI HOOMAN

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Journal: Iranian Journal of Field Crop Science Year:2017 | Volume:48 | Issue:3 Page(s): 695-708

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Title

ANALYSIS OF GENETIC AND CYTOGENETIC VARIATIONS BETWEEN ALFALFA (MEDICAGO SATIVA L.) GENOTYPES IN IRAN

Author(s)

FARSHADFAR MOHSEN | BOLOORCHIAN FATEMEH | SAFARI HOOSHMAND | SHIRVANI HOOMAN | Issue Writer Certificate

Keywords

GENETIC DIVERSITYQ2

KARYOTYPEQ3

ISSRQ1

MEDICAGO SATIVA L.Q1

Abstract

Alfalfa (Medicago sativa L.) is one of the important forage crops producing highly nutritious biomass and adaptability. In this study the GENETIC DIVERSITY among 19 genotypes of Medicago sativa was evaluated based on the molecular and cytogenetic markers. Molecular studies were carried out based on eight Inter Simple Sequence Repeat (ISSR) primers. Cytogenetic data were obtained by KARYOTYPE analysis with mitotic chromosomes. There were significant differences between cytogenetic characterictics (P£0.01). The genotypes ES-211, ES-027, ES 037, ES-065, and ES-199 had the greatest chromatin content and the highest asymmetrical value as well. The greatest amount of asymmetry index (AI) belonged to ES211, E119 while the least value of AI was ES037. The genotype ES119 had the greatest CVCL, Mean centromeric asymmetry (MCA), r-value and relative length (RL%) characterictics. Analysis of banding patterns of eight ISSR primers revealed 29 polymorphic bands. A total of 29 polymorphic fragments were scored and the average of polymorphic information content (PIC) was 0.32. The number of polymorphic fragments for each primer varied from three to six with mean of 4.62 polymorphic fragments per primer. The mean polymorphic percentage was 86%. The Cluster analysis of molecular data classified all 19 genotypes into three different groups. According to the karyotypic characterictics, all genotypes were grouped to three clusters as well.

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APA: Copy

FARSHADFAR, MOHSEN, BOLOORCHIAN, FATEMEH, SAFARI, HOOSHMAND, & SHIRVANI, HOOMAN. (2017). ANALYSIS OF GENETIC AND CYTOGENETIC VARIATIONS BETWEEN ALFALFA (MEDICAGO SATIVA L.) GENOTYPES IN IRAN. IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), 48(3 ), 695-708. SID. https://sid.ir/paper/153420/en

Vancouver: Copy

FARSHADFAR MOHSEN, BOLOORCHIAN FATEMEH, SAFARI HOOSHMAND, SHIRVANI HOOMAN. ANALYSIS OF GENETIC AND CYTOGENETIC VARIATIONS BETWEEN ALFALFA (MEDICAGO SATIVA L.) GENOTYPES IN IRAN. IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES)[Internet]. 2017;48(3 ):695-708. Available from: https://sid.ir/paper/153420/en

IEEE: Copy

MOHSEN FARSHADFAR, FATEMEH BOLOORCHIAN, HOOSHMAND SAFARI, and HOOMAN SHIRVANI, “ANALYSIS OF GENETIC AND CYTOGENETIC VARIATIONS BETWEEN ALFALFA (MEDICAGO SATIVA L.) GENOTYPES IN IRAN,” IRANIAN JOURNAL OF FIELD CROP SCIENCE (IRANIAN JOURNAL OF AGRICULTURAL SCIENCES), vol. 48, no. 3 , pp. 695–708, 2017, [Online]. Available: https://sid.ir/paper/153420/en

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