Ultrasonic waves effect on germination, α amylase activity, antioxidant enzymes, sugars and chlorophyll on Echinacea purpurea L.

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

RASOULI F.; GHOLIPOOR M.; JAHANBIN K.; ASGHARI H.R.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Plant Production Research Year:2019 | Volume:26 | Issue:3 Page(s): 21-36

Download Full-Text

Persian Verion

View:

551

Download:

Cites:

Information Journal Paper

Title

Ultrasonic waves effect on germination, α amylase activity, antioxidant enzymes, sugars and chlorophyll on Echinacea purpurea L.

Author(s)

RASOULI F. | GHOLIPOOR M. | JAHANBIN K. | ASGHARI H.R. | Issue Writer Certificate

Keywords

ChlorophyllQ2

Seedling dry weightQ1

Seed Reserve Use EfficiencyQ1

SugarsQ1

Abstract

Background and Objectives: coneflower seeds germination is low and non-uniform. One reasons for low germination of coneflower seeds is low permeability of internal membranes. Seed priming is very simple procedures which can improve germination and seedling establishment. One of the biophysical ways of seed priming is pretreatment of seeds with ultrasound waves. Continuing wave beating increases the permeability, enhances water absorption and temperature of seeds. Increasing imbibition rate accompanies with speedy changes in seed metabolism. This experiment was aimed to study the ultrasonic wave’ s effect on germination, α amylase activity, Sugars, Chlorophyll, hydrogen peroxide and some antioxidant enzymes in germination and seedling stages of coneflower. Materials and Methods: The experiment was conducted as completely randomized design with 5 treatments and 4 replications in Agriculture Faculty of Shahrood University of Technology in 2017. Treatments were ultra-sonic wave durations including 0, 10, 15, 20 and 25 min by ultra-sonication device, Then germination percentage, germination rate (R50), Seed Reserve Use Efficiency (SRUE), Seedling dry weight, α amylase activity, total sugar, reducing Sugars, non-reducing Sugars, ratio of non-reducing to reducing Sugars, electrical conductivity, hydrogen peroxide, CAT, GPX, PPO, G-ST and Chl a and b measured. Results: The highest amount of germination percentage from 15 min and the maximum of R50 from 15 and 25 min seed pretreatment by ultra-sonic waves obtained. All treatments increased α amylase activity, total sugar and non-reducing Sugars concentration compared to the control. The highest amount of the SRUE in 15 and 10 min pretreatment with mean about 0. 80 mg and the lowest of it in control treatment with mean of 0. 59 mg in seed observed. The highest amount of Seedling dry weight in 15 min with average of 0. 665 g and the lowest of it in control treatment with average of 0. 0467 g obtained. Reducing Sugars in treatment of 25 min with average of 405. 5 μ g per g was at first level and control with mean of 318 μ g per g was at second level. The highest amount of non-reducing Sugars in treatment of 15 min with average of 43. 5 and the lowest of it in control with average of 16. 9 μ g per g of FW obtained. Seed pretreatment with ultrasonic wave cause to increase electrical conductivity and hydrogen peroxide concentration in 15, 20 and 25 min than to control. In order to eliminate hydrogen peroxide CAT, GPX, GST and PPO activity in most treatments increased. The highest amount of GPX activity in 15 and 25 min obtained. The highest amount of CAT and PPO activity in 15 min obtained. The highest amount of GST activity in 15, 20 and 25 min obtained. Conclusion: Coneflower seeds pretreatment with ultrasonic waves increased α-amylase enzyme activity and then Sugars concentration in most treatment increased. Continuous impact of ultrasound waves on seeds increased electrical leakage of seeds membranes and hydrogen peroxide concentration in 15, 20 and 25 minutes, in order to eliminate this stress CAT, GPX, GST and PPO activity in most treatment increased, which ultimately ultrasonic waves increased germination percentage, R50, Seedling dry weight and SRUE in most treatment compared to control.

Cites

No record.

References

No record.

Cite

APA: Copy

RASOULI, F., GHOLIPOOR, M., JAHANBIN, K., & ASGHARI, H.R.. (2019). Ultrasonic waves effect on germination, α amylase activity, antioxidant enzymes, sugars and chlorophyll on Echinacea purpurea L.. JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES), 26(3 ), 21-36. SID. https://sid.ir/paper/155741/en

Vancouver: Copy

RASOULI F., GHOLIPOOR M., JAHANBIN K., ASGHARI H.R.. Ultrasonic waves effect on germination, α amylase activity, antioxidant enzymes, sugars and chlorophyll on Echinacea purpurea L.. JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES)[Internet]. 2019;26(3 ):21-36. Available from: https://sid.ir/paper/155741/en

IEEE: Copy

F. RASOULI, M. GHOLIPOOR, K. JAHANBIN, and H.R. ASGHARI, “Ultrasonic waves effect on germination, α amylase activity, antioxidant enzymes, sugars and chlorophyll on Echinacea purpurea L.,” JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES), vol. 26, no. 3 , pp. 21–36, 2019, [Online]. Available: https://sid.ir/paper/155741/en

Related Journal Papers