SEROLOGICAL AND MOLECULAR DETECTION OF WHEAT STREAK MOSAIC VIRUS (WSMV) IN CEREAL FIELDS OF GOLESTAN PROVINCE, NORTHERN IRAN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KHADIVAR R.S.; NASR ELAHNEZHAD S.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Plant Production Research Year:2009 | Volume:16 | Issue:4 Page(s): 137-148

Download Full-Text

Persian Verion

View:

1,030

Download:

Cites:

Information Journal Paper

Title

SEROLOGICAL AND MOLECULAR DETECTION OF WHEAT STREAK MOSAIC VIRUS (WSMV) IN CEREAL FIELDS OF GOLESTAN PROVINCE, NORTHERN IRAN

Author(s)

KHADIVAR R.S. | NASR ELAHNEZHAD S. | Issue Writer Certificate

Keywords

WHEAT STREAK MOSAIC VIRUSQ3

ELISAQ2

RT-PCRQ3

ECHINOCHLOA COLONUMQ3

Abstract

In recent years, symptoms of a viral disease including streak mosaic, leaf blade malformation, and thickness (scrawniness) of raceme have been observed in the vast areas of grain fields in Golestan province. In order to identify the causes of this disease, detection of WHEAT STREAK MOSAIC VIRUS (WSMV) in cultivated hosts including wheat, dominant weeds of fields involving barnyardgrass (Echinochloa crus-galli L.), johnsongrass (Sorghum halepense), Setaria glauca, Digitaria sanguinalis, Bromus spp., and ECHINOCHLOA COLONUM is done with mentioned symptoms. In the agricultural year of 2007, 700 samples of these hosts in the fields of the province, suspected of the disease, were prepared. These Samples were analyzed by indirect ELISA using WSMV polyclonal antiserum and a number of the infected samples were tested by PCR using WSMV specific primers involving RCF1, WSM1, WSMF10 and WSMF13 that were prepared from the 5' area of the virus’s protein capsid. Results of indirect ELISA confirmed the presence of this virus in the Digitaria sanguinalis and ECHINOCHLOA COLONUM, for first time. Positive reactions in the RT-PCR test with WSMV specific primers also showed the presence of this virus in only two hosts, Digitaria sanguinalis and ECHINOCHLOA COLONUM. We have no information about the scope of the damage in the fields of the province. In this research although in some cases wheat fields and wild weed in the fields and neighboring gardens showed the symptoms including mosaic, leaf curl, dwarfing and decrease of the growth, but the presence of this virus has not been detected in most cases. Investigation to detect the virus in native hosts involving wheat is continued in this province.

Cites

No record.

References

No record.

Cite

APA: Copy

KHADIVAR, R.S., & NASR ELAHNEZHAD, S.. (2009). SEROLOGICAL AND MOLECULAR DETECTION OF WHEAT STREAK MOSAIC VIRUS (WSMV) IN CEREAL FIELDS OF GOLESTAN PROVINCE, NORTHERN IRAN. JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES), 16(4), 137-148. SID. https://sid.ir/paper/155989/en

Vancouver: Copy

KHADIVAR R.S., NASR ELAHNEZHAD S.. SEROLOGICAL AND MOLECULAR DETECTION OF WHEAT STREAK MOSAIC VIRUS (WSMV) IN CEREAL FIELDS OF GOLESTAN PROVINCE, NORTHERN IRAN. JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES)[Internet]. 2009;16(4):137-148. Available from: https://sid.ir/paper/155989/en

IEEE: Copy

R.S. KHADIVAR, and S. NASR ELAHNEZHAD, “SEROLOGICAL AND MOLECULAR DETECTION OF WHEAT STREAK MOSAIC VIRUS (WSMV) IN CEREAL FIELDS OF GOLESTAN PROVINCE, NORTHERN IRAN,” JOURNAL OF PLANT PRODUCTION (JOURNAL OF AGRICULTURAL SCIENCES AND NATURAL RESOURCES), vol. 16, no. 4, pp. 137–148, 2009, [Online]. Available: https://sid.ir/paper/155989/en

Related Journal Papers