THE EFFECT OF HORMONE TYPE AND BASIC CULTURAL MEDIA ON MULTIPLICATION AND ROOTING OF HERBAL SPECIES OF RIBES KHORASANICUM

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DARROUDI H.; AKBARINIA M.; SAFARNEJAD A.; HOSSEINI S.M.; HAJIAN SHAHRI M.

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Journal: Journal of Wood and Forest Science and Technology Year:2016 | Volume:23 | Issue:3 Page(s): 1-22

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Title

THE EFFECT OF HORMONE TYPE AND BASIC CULTURAL MEDIA ON MULTIPLICATION AND ROOTING OF HERBAL SPECIES OF RIBES KHORASANICUM

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Keywords

RIBES KHORASANICUMQ1

MICROPROPAGATIONQ2

BAPQ1

ROOT LENGHTQ1

Abstract

Background and objectives: RIBES KHORASANICUM is a deciduous woody shrub form and is one of the most valuable species belongs to the monogenus family of Grossulariaceae. Ribes is a genus has about 150 species of flowering plants native throughout the temperate regions of the Northern Hemisphere. Because of the nonnormative utilization, the utility of fruits, high palatability, livestock grazing, restricted ecological range, high ecological demands and sexual reproduction difficulties (mainly regenerates by the vegetative method by rhizome) facing risk of extinction. One of the possible methods of protection of the endangered taxon is multiplying and conservation of plants with help in vitro cultures.Materials and methods: Axillary and apical buds of the branches obtained from endemic plants in Hezarmasjed Mountains were used as initial explants. In this research effect of two cytokinins, BAP and Kinetin in presence or absence of auxin on proliferation coefficient and growth of multiplied shoots were investigated. The concentrations (0, 0.5, 1, 3 and 5) mg/l of each of cytokinins in the presence or absence of 0.05 mg IBA were studied. Furthermore, effect of nutrient concentration and sucrose concentration of cultural media, IBA and BAP hormones on rooting Percent and plantlet root length was investigated. Used treatment for root induction investigations was the concentrations of (0, 0.1, 0.25, 0.5, 1, 2 and 5) mg/l of IBA hormone in presence or absence of 0.1 mg/l of BAP hormone in MS and MS/2 media.Results: Results showed that, BAP hormone has the better shoot multiplication index than kinetin, considering the high rate of proliferation and higher shoots, the most suitable culture media for multiplication was MS media supplemented by 1 mg/l BAP. According to the treatments evaluated, and the average percentage of rooting and root length of plant lets, for root induction the best media was, modified MS media which sucrose concentration reduced to 15 g/l, and supplemented with0.5 to 2 mg/l of IBA. Addition of 0.1 mg per liter BAP decreased the percentage of rooting and root length. The longest root of plantlets was obtained from the modified MS media supplemented with 2mg/l IBA. Plantlets were successfully transferred from tissue culture vessels to ex vitro conditions with about 86 percent survival.Conclusion: According to high multiplication index and successful acclimatization of tissue cultured plantlets, the tissue culture can be used as an alternative with seeds and cuttings propagation methods to maintain and expand this valuable herbal shrub.

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APA: Copy

DARROUDI, H., AKBARINIA, M., SAFARNEJAD, A., HOSSEINI, S.M., & HAJIAN SHAHRI, M.. (2016). THE EFFECT OF HORMONE TYPE AND BASIC CULTURAL MEDIA ON MULTIPLICATION AND ROOTING OF HERBAL SPECIES OF RIBES KHORASANICUM. JOURNAL OF WOOD AND FOREST SCIENCE AND TECHNOLOGY, 23(3), 1-22. SID. https://sid.ir/paper/156782/en

Vancouver: Copy

DARROUDI H., AKBARINIA M., SAFARNEJAD A., HOSSEINI S.M., HAJIAN SHAHRI M.. THE EFFECT OF HORMONE TYPE AND BASIC CULTURAL MEDIA ON MULTIPLICATION AND ROOTING OF HERBAL SPECIES OF RIBES KHORASANICUM. JOURNAL OF WOOD AND FOREST SCIENCE AND TECHNOLOGY[Internet]. 2016;23(3):1-22. Available from: https://sid.ir/paper/156782/en

IEEE: Copy

H. DARROUDI, M. AKBARINIA, A. SAFARNEJAD, S.M. HOSSEINI, and M. HAJIAN SHAHRI, “THE EFFECT OF HORMONE TYPE AND BASIC CULTURAL MEDIA ON MULTIPLICATION AND ROOTING OF HERBAL SPECIES OF RIBES KHORASANICUM,” JOURNAL OF WOOD AND FOREST SCIENCE AND TECHNOLOGY, vol. 23, no. 3, pp. 1–22, 2016, [Online]. Available: https://sid.ir/paper/156782/en

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