DIRECT REGENERATION OF BIRCH (BETULA LITWINOWII) BYIN VITRO CULTURE NODAL EXPLANTS

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NAZARI J.; PAYAMNOOR V.; ALIZADEH M.; GHASEMI YAZDI K.

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Journal: Journal of Wood and Forest Science and Technology Year:2013 | Volume:20 | Issue:3 Page(s): 183-192

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Title

DIRECT REGENERATION OF BIRCH (BETULA LITWINOWII) BYIN VITRO CULTURE NODAL EXPLANTS

Author(s)

NAZARI J. | PAYAMNOOR V. | ALIZADEH M. | GHASEMI YAZDI K. | Issue Writer Certificate

Keywords

BETULA LITWINOWIIQ2

TISSUE CULTUREQ2

MICROPROPAGATIONQ2

STERILIZATIONQ2

Abstract

Birch (Betula sp.) is considered as a survivor of Caspian primary forests and one of the extinct forest trees in Iran. In our country, Birch species is endangered because of limited distribution range, unfavourable habitat conditions, lack of regeneration in natural habitat and inability to reproduce sexually in natural condition. Therefore, using the technique of in vitro is very important to support this species. The objective of this study is to optimize culture medium and surface STERILIZATION treatments for B. litwinowii micro-propagation, that is one of known Birch by use of nodal explants. Nodal explants were collected randomly from 4-5 years old of birch seedlings and sterilised by applying different treatments in both MS and WPM medium with various concentrations of BAP and NAA hormones were cultured. The obtained data were analyzed based on completely randomized design using SPSS and MSTATC software. The results revealed that HgCl2 (0.1% for 7 min) was the best treatment for surface STERILIZATION of nodal explants. The MS and WPM media supplemented with various concentrations of hormones were suitable for culture establishment of nodal explants. More than 70% of single lateral bud nodal in both mediums becomes to leaves. Finally, 10% of nodal explants are able to produce shoot and roots in WPM medium.

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APA: Copy

NAZARI, J., PAYAMNOOR, V., ALIZADEH, M., & GHASEMI YAZDI, K.. (2013). DIRECT REGENERATION OF BIRCH (BETULA LITWINOWII) BYIN VITRO CULTURE NODAL EXPLANTS. JOURNAL OF WOOD AND FOREST SCIENCE AND TECHNOLOGY, 20(3), 183-192. SID. https://sid.ir/paper/156790/en

Vancouver: Copy

NAZARI J., PAYAMNOOR V., ALIZADEH M., GHASEMI YAZDI K.. DIRECT REGENERATION OF BIRCH (BETULA LITWINOWII) BYIN VITRO CULTURE NODAL EXPLANTS. JOURNAL OF WOOD AND FOREST SCIENCE AND TECHNOLOGY[Internet]. 2013;20(3):183-192. Available from: https://sid.ir/paper/156790/en

IEEE: Copy

J. NAZARI, V. PAYAMNOOR, M. ALIZADEH, and K. GHASEMI YAZDI, “DIRECT REGENERATION OF BIRCH (BETULA LITWINOWII) BYIN VITRO CULTURE NODAL EXPLANTS,” JOURNAL OF WOOD AND FOREST SCIENCE AND TECHNOLOGY, vol. 20, no. 3, pp. 183–192, 2013, [Online]. Available: https://sid.ir/paper/156790/en

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