SEQUENCING AND COMPARATIVE ANALYSIS OF FLAGELLIN GENES FLIA AND FLIB IN BOVINE CLOSTRIDIUM CHAUVOEI ISOLATES

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JABBARI A.R.; AZIZIAN KH.; ESMAELIZAD M.

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Journal: Iranian Journal of Veterinary Medicine Year:2016 | Volume:10 | Issue:1 Page(s): 27-32

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Title

SEQUENCING AND COMPARATIVE ANALYSIS OF FLAGELLIN GENES FLIA AND FLIB IN BOVINE CLOSTRIDIUM CHAUVOEI ISOLATES

Author(s)

JABBARI A.R. | AZIZIAN KH. | ESMAELIZAD M. | Issue Writer Certificate

Keywords

CLOSTRIDIUM CHAUVOEIQ1

FLAGELLINQ1

POLYMORPHISMQ1

Abstract

BACKGROUND: CLOSTRIDIUM CHAUVOEI is the etiological agent of blackleg as an endogenous infection in cattle. Flagella have been known to play a critical role in the protective immunity of animals to clostridial infections. C. chauvoei has two copies offli C gene, namely fliA and fliB. OBJECTIVES: The aim of this study was the determination and nucleotide sequence analysis of both copies offli C genes in vaccinal strain and Iranian C. chauvoeiisolates.METHODS: Six specific primers for amplification offliA, fliB, and FLAGELLIN (fli C) genes were designed by Oligo software. Polymerase chain reaction was performed to amplify a fragment of 700 bp for both copies of FLAGELLIN (fliA and fliB) genes. The nucleotide percentage identity and divergence among isolates were deduced using BlAST and MegAlign softwares.RESULTS: It was found that divergence infliB was more than fliA by sequence alignment analysis. Six highly conserve regions, thirty-one SNPs and 13 amino acid POLYMORPHISMs were found infli C gene (between fliA and fliB sequences) of Iranian C. chauvoei isolates. In comparative analysis, genomic similarity of thefli A and fliB genes between the vaccinal strain and examined field isolates was proved to be as high as 97.3 % and 98.2%, respectively.CONCLUSIONS: The fliC copies were identified as excellent biomarkers to study the molecular epidemiology and strain diversity amongC. chauvoei isolates. The existence of genetic variation between two alleles offli C gene in C. chauvoei is reported for the first time in Iran. In spite of some genetic variations, the immunologic cross protection test showed a high protection power of the local vaccine (produced by Razi Institute) against homologous and heterologous challenge.

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APA: Copy

JABBARI, A.R., AZIZIAN, KH., & ESMAELIZAD, M.. (2016). SEQUENCING AND COMPARATIVE ANALYSIS OF FLAGELLIN GENES FLIA AND FLIB IN BOVINE CLOSTRIDIUM CHAUVOEI ISOLATES. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), 10(1), 27-32. SID. https://sid.ir/paper/157875/en

Vancouver: Copy

JABBARI A.R., AZIZIAN KH., ESMAELIZAD M.. SEQUENCING AND COMPARATIVE ANALYSIS OF FLAGELLIN GENES FLIA AND FLIB IN BOVINE CLOSTRIDIUM CHAUVOEI ISOLATES. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)[Internet]. 2016;10(1):27-32. Available from: https://sid.ir/paper/157875/en

IEEE: Copy

A.R. JABBARI, KH. AZIZIAN, and M. ESMAELIZAD, “SEQUENCING AND COMPARATIVE ANALYSIS OF FLAGELLIN GENES FLIA AND FLIB IN BOVINE CLOSTRIDIUM CHAUVOEI ISOLATES,” IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), vol. 10, no. 1, pp. 27–32, 2016, [Online]. Available: https://sid.ir/paper/157875/en

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