THE PRODUCTION OF MOLECULAR CLONES OF TOXOPLASMA GONDII-DERIVED HEAT SHOCK PROTEIN 70 KDA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SEYED TABAEI S.J.; DALIMI A.A.H.; KAZEMI BAHRAM; SADRAEI J.; GHAFARIFARD F.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Veterinary Medicine Year:2010 | Volume:4 | Issue:1 Page(s): 41-44

Download Full-Text

Persian Verion

View:

784

Download:

Cites:

Information Journal Paper

Title

THE PRODUCTION OF MOLECULAR CLONES OF TOXOPLASMA GONDII-DERIVED HEAT SHOCK PROTEIN 70 KDA

Author(s)

Keywords

TOXOPLASMA GONDIIQ3

CLONINGQ3

SEQUENCING;HEAT SHOCK PROTEIN 70Q3

Abstract

Toxoplasmosis is a common and widespread infection in humans and many other species of warm-blooded animals. TOXOPLASMA GONDII-derived heat shock protein 70 (Hsp70) may play an important role in the virulence of TOXOPLASMA GONDII (T gondii). In the present study, T gondii Hsp70 was amplified by polymerase chain reaction (PCR) from the DNA of the T gondii tachyzoite RH strain through the use of specific primers with Xho 1 and Xbal restriction sites. The purified DNA fragment of the T gondii Hsp70 gene was subcloned into the Xhol and Xbal-digested eukaryotic expression vector, pcDNA3, and subsequently transformed into TOP 10 chemically competent cells. A 2004 base pair (bp) band of PCR product was observed on the 0.8% agarose gel. The cDNA was inserted into the pTZ57R/T vector and then subcloned successfully into the pcDNA3 eukaryotic expression plasmid vector. The sequence of this amplified gene showed up to 100% homology with the target gene according to the Genbank database (Accession no. U82281).

Cites

No record.

References

No record.

Cite

APA: Copy

SEYED TABAEI, S.J., DALIMI, A.A.H., KAZEMI, BAHRAM, SADRAEI, J., & GHAFARIFARD, F.. (2010). THE PRODUCTION OF MOLECULAR CLONES OF TOXOPLASMA GONDII-DERIVED HEAT SHOCK PROTEIN 70 KDA. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), 4(1), 41-44. SID. https://sid.ir/paper/157887/en

Vancouver: Copy

SEYED TABAEI S.J., DALIMI A.A.H., KAZEMI BAHRAM, SADRAEI J., GHAFARIFARD F.. THE PRODUCTION OF MOLECULAR CLONES OF TOXOPLASMA GONDII-DERIVED HEAT SHOCK PROTEIN 70 KDA. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)[Internet]. 2010;4(1):41-44. Available from: https://sid.ir/paper/157887/en

IEEE: Copy

S.J. SEYED TABAEI, A.A.H. DALIMI, BAHRAM KAZEMI, J. SADRAEI, and F. GHAFARIFARD, “THE PRODUCTION OF MOLECULAR CLONES OF TOXOPLASMA GONDII-DERIVED HEAT SHOCK PROTEIN 70 KDA,” IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), vol. 4, no. 1, pp. 41–44, 2010, [Online]. Available: https://sid.ir/paper/157887/en

Related Journal Papers