Osteogenic Differentiation of Mesenchymal Stem Cells Via Osteoblast-Imprinted Substrate: In Vitro and In Vivo Evaluation in Rat Model

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GHOLAMI HOSSEIN; MARDJANMEHR SEYED HOSSEIN; DEHGHAN MOHAMMAD MEHDI; BONAKDAR SHAHIN; Farzad Mohajeri Saeed

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Journal: Iranian Journal of Veterinary Medicine Year:2019 | Volume:13 | Issue:3 Page(s): 260-269

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Title

Osteogenic Differentiation of Mesenchymal Stem Cells Via Osteoblast-Imprinted Substrate: In Vitro and In Vivo Evaluation in Rat Model

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Keywords

Adipose derived mesenchymal stem cells (ADSCs)Q1

alkaline phosphataseQ1

calvarial defectQ1

cell-imprinted substrateQ1

osteogenic differentiationQ1

Abstract

BACKGROUND: Stem cells have great effects in clinical cell-based therapy. Accordingly, controlling the behavior and directing the fate of stem cells cultured in the laboratory is an important issue. OBJECTIVES: The aim of this study was to evaluate osteogenic properties of adipose derived mesenchymal stem cells (ADSCs) which differentiated toward osteogenic linage by osteoblast-imprinted substrate. METHODS: Rat ADSCs seeded on osteoblast-imprinted substrates, alkaline phosphatase (ALP) were measured in cellular supernatant of days 7 and 14. Alizarin Red staining of mineral matrix production in day 17 was performed. In order for in vivo evaluation, after seeding differentiated cells on a simple collagen scaffold, samples were implanted in an 8mm critical size calvarial defect. After 4 weeks defect site was harvested and prepared for histopathological examination. RESULTS: ALP in both time points was significantly more than in undifferentiated ADSCs. (p<0. 05). Alizarin red staining of differentiated cells showed a great production of mineral matrix nodules in cell culture plate. Histopathological investigations revealed greater amount of new bone formation and mostly in the center of defect, indicate osteoinductive effect of differentiated cells in vivo. CONCLUSIONS: The osteoblast-imprinted substrate can mimic the topography and shape of natural osteoblast, which can mechanically direct ADSCs toward osteogenesis.

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APA: Copy

GHOLAMI, HOSSEIN, MARDJANMEHR, SEYED HOSSEIN, DEHGHAN, MOHAMMAD MEHDI, BONAKDAR, SHAHIN, & Farzad Mohajeri, Saeed. (2019). Osteogenic Differentiation of Mesenchymal Stem Cells Via Osteoblast-Imprinted Substrate: In Vitro and In Vivo Evaluation in Rat Model. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), 13(3 ), 260-269. SID. https://sid.ir/paper/158005/en

Vancouver: Copy

GHOLAMI HOSSEIN, MARDJANMEHR SEYED HOSSEIN, DEHGHAN MOHAMMAD MEHDI, BONAKDAR SHAHIN, Farzad Mohajeri Saeed. Osteogenic Differentiation of Mesenchymal Stem Cells Via Osteoblast-Imprinted Substrate: In Vitro and In Vivo Evaluation in Rat Model. IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH)[Internet]. 2019;13(3 ):260-269. Available from: https://sid.ir/paper/158005/en

IEEE: Copy

HOSSEIN GHOLAMI, SEYED HOSSEIN MARDJANMEHR, MOHAMMAD MEHDI DEHGHAN, SHAHIN BONAKDAR, and Saeed Farzad Mohajeri, “Osteogenic Differentiation of Mesenchymal Stem Cells Via Osteoblast-Imprinted Substrate: In Vitro and In Vivo Evaluation in Rat Model,” IRANIAN JOURNAL OF VETERINARY MEDICINE (INTERNATIONAL JOURNAL OF VETERINARY RESEARCH), vol. 13, no. 3 , pp. 260–269, 2019, [Online]. Available: https://sid.ir/paper/158005/en

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