REPRODUCTION OF SINORHIZOBIUM MELILOTI BACTERIA IN SOME INEXPENSIVE CULTURE MEDIA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GHORBANI SH.; SADAT EBRAHIMI S.E.; KHAVAZI K.; ARZANESH M.H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Soil Research Year:2005 | Volume:19 | Issue:2 Page(s): 203-214

Download Full-Text

Persian Verion

View:

1,175

Download:

Cites:

Information Journal Paper

Title

REPRODUCTION OF SINORHIZOBIUM MELILOTI BACTERIA IN SOME INEXPENSIVE CULTURE MEDIA

Author(s)

GHORBANI SH. | SADAT EBRAHIMI S.E. | KHAVAZI K. | ARZANESH M.H. | Issue Writer Certificate

Keywords

MALT SPROUTQ4

CHEESE WHEYQ4

SINORHIZOBIUM MELILOTIQ3

Abstract

Reproduction of selected strains is one of the most expensive stages in the process of inoculum production. Therefore, for large scale industrial production of rhizobiums, efforts are made to use the by-products of food industry plants directly or in combination with some other ingredients as culture media. In this experiment multiplication of S. meliloti in three culture media, namely MALT SPROUT extract, dehydrated CHEESE WHEY, and the chemical base medium of yeast extract mannitol along with three nitrogen sources: ammonium chloride, potassium nitrate, and yeast extract; four carbon sources: mannitol, glucose, galactose, and sucrose at two pH levels of 6.8 and 7.0 was investigated. The experiment was designed as a randomized complete block factorial test with three replications. Isolates of SM-11 (S. meliloti) with population densities of 2×l05 cells/ml were transferred to 100 ml Erlenmeyer flasks containing the culture media mentioned before, followed by making plate counts on YMA, growth medium containing Congo-red. After two days of bacterial growth, the results of the experiment showed that the bacterial populations reached a level of 7.94×109 cells/ml in the malt culture containing mannitol as a carbon source and yeast extract as a nitrogen source with the pH of the medium adjusted to 7.0, while the population density in the same medium, but containing sucrose and potassium nitrate adjusted to pH 6.8 was measured to be 4.5× 109 cells/ml, as compared with 5.24×109 cells/ml for the standard YMB culture medium. Likewise, the population density of the bacteria in the chemical base culture at pH of 6.8, was 2.07×109 cell/ml after 3 days and in the same medium containing sucrose and yeast extract at pH of 7.0 was measured to be 5.24×108 cells/ml. These results indicate that, to reproduce this isolate inexpensively on industrial scales for inoculum production, the MALT SPROUT extract growth medium containing sucrose and potassium nitrate is recommendable.

Cites

No record.

References

Cite

APA: Copy

GHORBANI, SH., SADAT EBRAHIMI, S.E., KHAVAZI, K., & ARZANESH, M.H.. (2005). REPRODUCTION OF SINORHIZOBIUM MELILOTI BACTERIA IN SOME INEXPENSIVE CULTURE MEDIA. IRANIAN JOURNAL OF SOIL AND WATERS SCIENCES, 19(2), 203-214. SID. https://sid.ir/paper/15993/en

Vancouver: Copy

GHORBANI SH., SADAT EBRAHIMI S.E., KHAVAZI K., ARZANESH M.H.. REPRODUCTION OF SINORHIZOBIUM MELILOTI BACTERIA IN SOME INEXPENSIVE CULTURE MEDIA. IRANIAN JOURNAL OF SOIL AND WATERS SCIENCES[Internet]. 2005;19(2):203-214. Available from: https://sid.ir/paper/15993/en

IEEE: Copy

SH. GHORBANI, S.E. SADAT EBRAHIMI, K. KHAVAZI, and M.H. ARZANESH, “REPRODUCTION OF SINORHIZOBIUM MELILOTI BACTERIA IN SOME INEXPENSIVE CULTURE MEDIA,” IRANIAN JOURNAL OF SOIL AND WATERS SCIENCES, vol. 19, no. 2, pp. 203–214, 2005, [Online]. Available: https://sid.ir/paper/15993/en

Related Journal Papers