HRGFP TRANSFECTION INTO ZEBRAFISH ZF4 CELL LINE USING TWO RECOMBINANT PROTEINS VIRD2 & VIRE2

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GHOVATI NEDA; FARAHMAND HAMID; ALIZADEH HOUSHANG

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ISSN: 2588-4824

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Paper Information

Journal: Journal of Fisheries Year:2013 | Volume:66 | Issue:2 Page(s): 233-239

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Title

HRGFP TRANSFECTION INTO ZEBRAFISH ZF4 CELL LINE USING TWO RECOMBINANT PROTEINS VIRD2 & VIRE2

Author(s)

GHOVATI NEDA | FARAHMAND HAMID | ALIZADEH HOUSHANG | Issue Writer Certificate

Keywords

CELL LINEQ2

GENE TRANSFERQ2

HRGFPQ2

RECOMBINANT PROTEINQ2

ZEBRAFISHQ2

Abstract

Import of DNA into fish genome is generally inefficient. Therefore, one of the current challenges in fish tarnsgenesis is the development of efficient DNA delivery systems. Here we test using two bacterial proteins to target DNA to ZEBRAFISH ZF4 CELL LINE genome. Agrobacterium tumefaciens, a soil bacterium, efficiently transfers DNA as a nucleoprotein complex to plant cells genome. Agrobacterium proteins VirD2 and VirE2 perform important functions in this process. We reconstitute this synthetic complexes consisting of the bacterial proteins VirD2, VirE2, and single-stranded DNA (ssDNA) in vitro, to test the potential of this approach to enhance GENE TRANSFER efficiency to ZEBRAFISH ZF4 CELL LINE. Our result revealed that VirD2 and VirE2 improved the efficiency of DNA integration into ZEBRAFISH ZF4 CELL LINE genome.

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APA: Copy

GHOVATI, NEDA, FARAHMAND, HAMID, & ALIZADEH, HOUSHANG. (2013). HRGFP TRANSFECTION INTO ZEBRAFISH ZF₄ CELL LINE USING TWO RECOMBINANT PROTEINS VIRD2 & VIRE2. JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES), 66(2), 233-239. SID. https://sid.ir/paper/161977/en

Vancouver: Copy

GHOVATI NEDA, FARAHMAND HAMID, ALIZADEH HOUSHANG. HRGFP TRANSFECTION INTO ZEBRAFISH ZF₄ CELL LINE USING TWO RECOMBINANT PROTEINS VIRD2 & VIRE2. JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES)[Internet]. 2013;66(2):233-239. Available from: https://sid.ir/paper/161977/en

IEEE: Copy

NEDA GHOVATI, HAMID FARAHMAND, and HOUSHANG ALIZADEH, “HRGFP TRANSFECTION INTO ZEBRAFISH ZF₄ CELL LINE USING TWO RECOMBINANT PROTEINS VIRD2 & VIRE2,” JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES), vol. 66, no. 2, pp. 233–239, 2013, [Online]. Available: https://sid.ir/paper/161977/en

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