Non-destructive Molecular Detection of Streptococcus agalactiae in Rainbow Trout (Oncorhynchus mykiss)

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Farid Mehrnoosh; Farid Mehrnoosh; MIRVAGHEFI ALIREZA; FARAHMAND HAMID; NEMATOLLAHI MOHAMMAD ALI

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Journal: Journal of Fisheries Year:2017 | Volume:70 | Issue:3 Page(s): 319-326

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Title

Non-destructive Molecular Detection of Streptococcus agalactiae in Rainbow Trout (Oncorhynchus mykiss)

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Keywords

Molecular diagnostic methodQ1

PCRQ1

MucusQ1

BloodQ1

DiseasesQ1

Abstract

The objective of this study was to evaluate a non-lethal sampling technique to detect Streptococcus agalactiae, the bacterial pathogen causing Streptococcosis, in the Blood and Mucus of the infected rainbow trout, Oncorhynchus mykiss. Detection was established by PCR protocol based on the scpB gene. Also biochemical identification method was set by API strepsystem and conventional bacteriological tests. The fish were infected by intraperitoneally (i. p. ) with 8×107 CFU/ml colony forming units of S. agalactiae. The PCR and biochemical method were positive for the Blood samples from 3th and 4th days after exposure up to the end of the experiment while the fish look healthy. Mucus samples in PCR assay and biochemical methods were not positive until the end of the experiment. All any biochemically positive results were positive in PCR protocol, but some PCR positive results were negative in biochemical method. PCR Sensitivity tests were done in fish tissues seeded with serial dilutions of pure culture of S. agalactiae. Mucus was not efficient when the bacteria count is low. This study revealed that a nonlethal Blood sampling can be used with high efficiency primers for PCR protocol to detect S. agalactiae in asymptomatic fish.

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APA: Copy

Farid, Mehrnoosh, Farid, Mehrnoosh, MIRVAGHEFI, ALIREZA, FARAHMAND, HAMID, & NEMATOLLAHI, MOHAMMAD ALI. (2017). Non-destructive Molecular Detection of Streptococcus agalactiae in Rainbow Trout (Oncorhynchus mykiss). JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES), 70(3 ), 319-326. SID. https://sid.ir/paper/162035/en

Vancouver: Copy

Farid Mehrnoosh, Farid Mehrnoosh, MIRVAGHEFI ALIREZA, FARAHMAND HAMID, NEMATOLLAHI MOHAMMAD ALI. Non-destructive Molecular Detection of Streptococcus agalactiae in Rainbow Trout (Oncorhynchus mykiss). JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES)[Internet]. 2017;70(3 ):319-326. Available from: https://sid.ir/paper/162035/en

IEEE: Copy

Mehrnoosh Farid, Mehrnoosh Farid, ALIREZA MIRVAGHEFI, HAMID FARAHMAND, and MOHAMMAD ALI NEMATOLLAHI, “Non-destructive Molecular Detection of Streptococcus agalactiae in Rainbow Trout (Oncorhynchus mykiss),” JOURNAL OF FISHERIES (IRANIAN JOURNAL OF NATURAL RESOURCES), vol. 70, no. 3 , pp. 319–326, 2017, [Online]. Available: https://sid.ir/paper/162035/en

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